Document Detail

Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation.
MedLine Citation:
PMID:  18923828     Owner:  NLM     Status:  MEDLINE    
A robust Saccharomyces cerevisiae strain has been widely applied in continuous and batch/fed-batch industrial fermentation. However, little is known about the molecular basis of fermentative behavior of this strain in the two realistic fermentation processes. In this paper, we presented comparative proteomic profiling of the industrial yeast in the industrial fermentation processes. The expression levels of most identified protein were closely interrelated with the different stages of fermentation processes. Our results indicate that, among the 47 identified protein spots, 17 of them belonging to 12 enzymes were involved in pentose phosphate, glycolysis, and gluconeogenesis pathways and glycerol biosynthetic process, indicating that a number of pathways will need to be inactivated to improve ethanol production. The differential expressions of eight oxidative response and heat-shock proteins were also identified, suggesting that it is necessary to keep the correct cellular redox or osmotic state in the two industrial fermentation processes. Moreover, there are significant differences in changes of protein levels between the two industrial fermentation processes, especially these proteins associated with the glycolysis and gluconeogenesis pathways. These findings provide a molecular understanding of physiological adaptation of industrial strain for optimizing the performance of industrial bioethanol fermentation.
Jing-Sheng Cheng; Bin Qiao; Ying-Jin Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-16
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  81     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2008-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  327-38     Citation Subset:  IM    
Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin, People's Republic of China.
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MeSH Terms
Enzymes / analysis
Ethanol / metabolism
Metabolic Networks and Pathways
Proteome / analysis*
Saccharomyces cerevisiae / chemistry*
Saccharomyces cerevisiae Proteins / analysis*
Reg. No./Substance:
0/Enzymes; 0/Proteome; 0/Saccharomyces cerevisiae Proteins; 64-17-5/Ethanol

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