| Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines. | |
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MedLine Citation:
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PMID: 18400291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polybrominated diphenyl ethers (PBDEs) constitute a class of flame retardants whose residues have markedly increased in fish and human tissues during the last decade. In particular, the levels of certain PBDE congeners in salmon have raised concern regarding potential risks associated with dietary PBDE exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro cell models. We conducted a comparative study of oxyradical production and cell injury in rainbow trout gill (RTgill-W1) and trout liver cells (RTL-W1) exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE 47), a predominant BDE residue found in fish tissues such as salmonids. Exposure to low micromolar concentrations of BDE 47 elicited a significant loss in RTgill-W1 and RTL-W1 cell viability as measured by alamarBlue assay. The dose-response of BDE toxicity differed among the two cell lines, with the RTL-W1 liver cells showing greater resistance to toxicity at lower BDE 47 doses, but a more dramatic loss of viability relative to gill cells when challenged with higher (50 microM) doses. The sensitivity of the trout liver cells at higher BDE 47 exposures was reflected by a higher basal production of oxygen radical production by 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence that was markedly enhanced in the presence of BDE 47, suggesting an overwhelming of trout liver cell antioxidant defense pathways. Collectively, our data indicate that RTgill-W1 and RTL-W1 liver cells are sensitive to BDE 47-mediated cell injury through a mechanism that may involve oxidative stress. Our data also provide an in vitro basis for potential tissue differences in BDE 47-mediated cell injury. |
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Authors:
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J Shao; M L Eckert; L E J Lee; E P Gallagher |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-02-26 |
Journal Detail:
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Title: Marine environmental research Volume: 66 ISSN: 0141-1136 ISO Abbreviation: Mar. Environ. Res. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-16 Completed Date: 2008-09-17 Revised Date: 2013-05-22 |
Medline Journal Info:
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Nlm Unique ID: 9882895 Medline TA: Mar Environ Res Country: England |
Other Details:
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Languages: eng Pagination: 7-8 Citation Subset: IM |
Affiliation:
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Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98105, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Survival / drug effects Gills / cytology, drug effects Liver / cytology, drug effects Oncorhynchus mykiss / physiology* Polybrominated Biphenyls / toxicity* Reactive Oxygen Species / metabolism* Toxicity Tests, Acute / methods Water Pollutants, Chemical / toxicity* |
| Grant Support | |
ID/Acronym/Agency:
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P42 ES004696/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polybrominated Biphenyls; 0/Reactive Oxygen Species; 0/Water Pollutants, Chemical; 0/tetrabrominated diphenyl ether 47 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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