Document Detail


Comparative label-free LC-MS/MS analysis of colorectal adenocarcinoma and metastatic cells treated with 5-fluorouracil.
MedLine Citation:
PMID:  22623418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A label-free mass spectrometric strategy was used to examine the effect of 5-fluorouracil (5-FU) on the primary and metastatic colon carcinoma cell lines, SW480 and SW620, with and without treatment. 5-FU is the most common chemotherapeutic treatment for colon cancer. Pooled biological replicates were analyzed by nanoLC-MS/MS and protein quantification was determined via spectral counting. Phenotypic and proteomic changes were evident and often similar in both cell lines. The SW620 cells were more resistant to 5-FU treatment, with an IC(50) 2.7-fold higher than that for SW480. In addition, both cell lines showed pronounced abundance changes in pathways relating to antioxidative stress response and cell adhesion remodeling due to 5-FU treatment. For example, the detoxification enzyme NQO1 was increased with treatment in both cell lines, while disparate members of the peroxiredoxin family, PRDX2 or PRDX5 and PRDX6, were elevated with 5-FU exposure in either SW480 or SW620, respectively. Cell adhesion-associated proteins CTNNB1 and RhoA showed decreased expression with 5-FU treatment in both cell lines. The differential quantitative response in the proteomes of these patient-matched cell lines to drug treatment underscores the subtle molecular differences separating primary and metastatic cancer cells.
Authors:
Kerry M Bauer; Paul A Lambert; Amanda B Hummon
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  12     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-29     Completed Date:  2012-10-17     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1928-37     Citation Subset:  IM    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / chemistry*,  drug therapy*,  metabolism
Antimetabolites, Antineoplastic / pharmacology*
Antioxidants
Cell Adhesion
Cell Line, Tumor
Chromatography, Liquid / methods
Colorectal Neoplasms / chemistry*,  drug therapy*,  metabolism
Fluorouracil / pharmacology*
Humans
Neoplasm Metastasis
Proteome / analysis*
Reproducibility of Results
Tandem Mass Spectrometry / methods
Grant Support
ID/Acronym/Agency:
T32 GM075762/GM/NIGMS NIH HHS; T32GM075762/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Antioxidants; 0/Proteome; U3P01618RT/Fluorouracil
Comments/Corrections

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