| Comparative label-free LC-MS/MS analysis of colorectal adenocarcinoma and metastatic cells treated with 5-fluorouracil. | |
| | |
MedLine Citation:
|
PMID: 22623418 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A label-free mass spectrometric strategy was used to examine the effect of 5-fluorouracil (5-FU) on the primary and metastatic colon carcinoma cell lines, SW480 and SW620, with and without treatment. 5-FU is the most common chemotherapeutic treatment for colon cancer. Pooled biological replicates were analyzed by nanoLC-MS/MS and protein quantification was determined via spectral counting. Phenotypic and proteomic changes were evident and often similar in both cell lines. The SW620 cells were more resistant to 5-FU treatment, with an IC(50) 2.7-fold higher than that for SW480. In addition, both cell lines showed pronounced abundance changes in pathways relating to antioxidative stress response and cell adhesion remodeling due to 5-FU treatment. For example, the detoxification enzyme NQO1 was increasedwith treatment in both cell lines, while disparate members of the peroxiredoxin family, PRDX2 or PRDX5 and PRDX6, were elevated with 5-FU exposure in either SW480 or SW620, respectively. Cell adhesion associated proteins CTNNB1 and RhoA showed decreased expression with 5-FU treatment in both cell lines. The differential quantitative response in the proteomes of these patient-matched cell lines to drug treatment underscores the subtle molecular differences separating primary and metastatic cancer cells. |
| | |
Authors:
|
Kerry M Bauer; Paul A Lambert; Amanda B Hummon |
Related Documents
:
|
170218 - Temperature-dependent properties of cells transformed by a thermosensitive mutant (ts-1... 6209558 - Induction of a step in carcinogenesis that is normally associated with mutagenesis by n... 7241668 - Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5. 4020928 - Transformation of erythrocytes to spherocytes following incubation with malignant cells. 11559818 - Inactivation of p21 by e1a leads to the induction of apoptosis in dna-damaged cells. 9845098 - Redox generation of nitric oxide to radiosensitize hypoxic cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-5-23 |
Journal Detail:
|
Title: Proteomics Volume: - ISSN: 1615-9861 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-5-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101092707 Medline TA: Proteomics Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
Department of Chemistry and Biochemistry, University of Notre Dame, 251 Nieuwland Science Hall, Notre Dame, IN, 46556, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cance...
Next Document: Chemo-Enzymatic Conversion of Glucose into 5-Hydroxymethylfurfural in Seawater.