Document Detail

Comparative kinetics of fatty acid-amino acid conjugate elicitor biosynthesis by midgut tissue microsomes of Lepidopterous caterpillar larvae.
MedLine Citation:
PMID:  21104884     Owner:  NLM     Status:  In-Process    
N-Linolenoyl-L-glutamine is one of several structurally similar fatty acid-amino acid conjugate (FAC) elicitors found in the oral secretions of Lepidopterous caterpillars and its biosynthesis is catalyzed by membrane-associated alimentary tissue enzyme(s). FAC elicitors comprise 17-hydroxylated or non-hydroxylated linolenic acid coupled with L-glutamine or L-glutamate by an amide bond. We demonstrate in vitro biosynthesis of N-linolenoyl-L-glutamine by Manduca sexta, Heliothis virescens, and Helicoverpa zea tissue microsomes. Comparison of N-linolenoyl-L-glutamine biosynthesis kinetics for these species suggests that concurrent biosynthesis and hydrolysis contribute to proportions of FAC elicitors found in their oral secretions. The apparent K(m) values for coupling of sodium linolenate were 8.75±0.79, 14.3±3.7 and 20.7±3.4 mM and V(max) values were 2.92±0.14, 6.81±1.2 and 4.95±0.55 nmol/min/mg protein for H. zea, H. virescens and M. sexta, respectively. The K(m) values for coupling of L-glutamine were 10.5±0.26, 22.3±2.0 and 18.9±2.4 mM and V(max) values were 1.78±0.21, 3.71±0.50 and 2.49±0.41 nmol/min/mg of protein for H. zea, H. virescens and M. sexta, respectively.
Cameron G Lait; Melanie J Lobaido; Amy J Wiester; Sarah Kossak; James H Tumlinson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  75     ISSN:  1520-6327     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  264-74     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley Periodicals, Inc.
Center for Chemical Ecology, Department of Entomology, The Pennsylvania State University, Agricultural Sciences & Industries Building, University Park, PA 16802, USA.
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