Document Detail

Comparative kinetic effects of Mn (II), Mg (II) and the ATP/ADP ratio on phosphoenolpyruvate carboxykinases from Anaerobiospirillum succiniciproducens and Saccharomyces cerevisiae.
MedLine Citation:
PMID:  17216567     Owner:  NLM     Status:  MEDLINE    
The kinetic affinity for CO(2) of phosphoenolpyruvate PEP(5) carboxykinase from Anaerobiospirillum succiniciproducens, an obligate anaerobe which PEP carboxykinase catalyzes the carboxylation of PEP in one of the final steps of succinate production from glucose, is compared with that of the PEP carboxykinase from Saccharomyces cerevisiae, which catalyzes the decarboxylation of oxaloacetate in one of the first steps in the biosynthesis of glucose. For the A. succiniciproducens enzyme, at physiological concentrations of Mn(2+) and Mg(2+), the affinity for CO(2) increases as the ATP/ADP ratio is increased in the assay medium, while the opposite effect is seen for the S. cerevisiae enzyme. The results show that a high ATP/ADP ratio favors CO(2) fixation by the PEP carboxykinase from A. succiniciproducens but not for the S. cerevisiae enzyme. These findings are in agreement with the proposed physiological roles of S. cerevisiae and A. succiniciproducens PEP carboxykinases, and expand recent observations performed with the enzyme isolated from Panicum maximum (Chen et al. (2002) Plant Physiology 128: 160-164).
Sergio Bazaes; Mauricio Toncio; Maris Laivenieks; J Gregory Zeikus; Emilio Cardemil
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The protein journal     Volume:  26     ISSN:  1572-3887     ISO Abbreviation:  Protein J.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-15     Completed Date:  2007-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101212092     Medline TA:  Protein J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-9     Citation Subset:  IM    
Departamento de Química, Universidad Metropolitana de Ciencias de la Educación, Avda. J. P. Alessandri 774, Santiago, Chile.
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MeSH Terms
Adenosine Diphosphate / chemistry*
Adenosine Triphosphate / chemistry*
Anaerobiospirillum / metabolism*
Carbon Dioxide / chemistry
Dose-Response Relationship, Drug
Magnesium / chemistry*
Manganese / chemistry*
Metals / chemistry
Models, Biological
Models, Chemical
Phosphoenolpyruvate Carboxykinase (ATP) / chemistry*
Saccharomyces cerevisiae / metabolism*
Species Specificity
Reg. No./Substance:
0/Ions; 0/Metals; 124-38-9/Carbon Dioxide; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 7439-95-4/Magnesium; 7439-96-5/Manganese; EC Carboxykinase (ATP)

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