Document Detail


Comparative intermediary metabolism of vegetative cells and microcysts of Myxococcus xanthus.
MedLine Citation:
PMID:  4302296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crude extracts of both vegetative cells and glycerol-induced microcysts of Myxococcus xanthus contained the following enzyme activities: phosphofructokinase, phosphoglucoisomerase, fructose-1,6-diphosphatase, fructosediphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphopyruvate carboxylase, citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase, and uridine diphosphate glucose pyrophosphorylase. With the exception of isocitrate dehydrogenase, which was present at a fivefold higher concentration in microcysts, all activities in extracts from both types of cells were essentially equal. Hexokinase and pyruvate kinase could not be detected in extracts from either type of cell. Microcysts metabolized acetate at a lower rate than did vegetative cells. Most of this decrease was reflected in a substantial decrease in ability of microcysts to oxidize acetate to CO(2). In addition, microcysts and vegetative cells showed a different distribution of (14)C-label from incorporated acetate.
Authors:
B F Watson; M Dworkin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  96     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 Nov 
Date Detail:
Created Date:  1969-02-04     Completed Date:  1969-02-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1465-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Bacteria / drug effects,  enzymology,  growth & development,  metabolism*
Carbon Isotopes
Carboxy-Lyases / metabolism
Cell-Free System
Citric Acid Cycle*
Fructose-Bisphosphatase / metabolism
Fructose-Bisphosphate Aldolase / metabolism
Glucose / metabolism
Glucose-6-Phosphate Isomerase / metabolism
Glucosephosphate Dehydrogenase / metabolism
Glycerol / pharmacology
Hexokinase / metabolism
Isocitrate Dehydrogenase / metabolism
Ketoglutaric Acids
Lyases / metabolism
Malate Dehydrogenase / metabolism
Nucleotidyltransferases / metabolism
Oxidoreductases / metabolism
Oxygen Consumption
Phosphofructokinase-1 / metabolism
Phosphoglucomutase / metabolism
Phosphogluconate Dehydrogenase / metabolism
Pyruvate Kinase / metabolism
Spores / metabolism
Succinate Dehydrogenase / metabolism
Chemical
Reg. No./Substance:
0/Acetates; 0/Carbon Isotopes; 0/Ketoglutaric Acids; 50-99-7/Glucose; 56-81-5/Glycerol; EC 1.-/Oxidoreductases; EC 1.1.1.37/Malate Dehydrogenase; EC 1.1.1.41/Isocitrate Dehydrogenase; EC 1.1.1.43/Phosphogluconate Dehydrogenase; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.3.99.1/Succinate Dehydrogenase; EC 2.7.1.1/Hexokinase; EC 2.7.1.11/Phosphofructokinase-1; EC 2.7.1.40/Pyruvate Kinase; EC 2.7.7.-/Nucleotidyltransferases; EC 3.1.3.11/Fructose-Bisphosphatase; EC 4.-/Lyases; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.2.13/Fructose-Bisphosphate Aldolase; EC 5.3.1.9/Glucose-6-Phosphate Isomerase; EC 5.4.2.2/Phosphoglucomutase
Comments/Corrections

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