| Comparative in vitro toxicity of seven zinc-salts towards neuronal PC12 cells. | |
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MedLine Citation:
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PMID: 19306923 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Currently much attention has been given to the neurotoxicity of zinc, yet little is known about the influence of the counterions present. Therefore, we investigated the influence of different Zn(2+)-salts (concentrations range 0.05-0.3 mM) on cell viability, ATP and glutathione concentration and caspase activation in differentiated PC12 cells as a model for neuronal cells. Generally, at concentrations of 0.05 mM most Zn(2+)-salts were not cytotoxic except for zinc-citrate. At concentrations between 0.1 and 0.3 mM Zn(2+) a significant decrease in GSH and ATP levels preceded cell death induced by all salts, except of zinc-histidinate. Zinc-citrate and zinc-sulphate turned out to be the most toxic salts particularly at low concentrations. Analyses of caspase 3/7 activity showed that dependent on the concentration and the type of the salt used cell death may show more or less signs of both, necrosis and apoptosis. Interestingly, the uptake of Zn(2+) from zinc-sulphate and zinc-citrate was significantly higher than that of other salts, implicating a correlation between uptake and toxicity. In conclusion, Zn(2+)-salts could be divided into three categories with high (zinc-citrate, zinc-sulphate), moderate (zinc-orotate, zinc-acetate, zinc-chloride(,) zinc-gluconate) and low cytotoxicity (zinc-histidinate). |
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Authors:
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Sanja Pavlica; Frank Gaunitz; Rolf Gebhardt |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-21 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-18 Completed Date: 2009-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 653-9 Citation Subset: IM |
Affiliation:
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Institut für Biochemie, Medizinische Fakultät, Universität Leipzig, Johannisallee 30, 04103 Leipzig, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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analysis Animals Caspases / physiology Cell Survival / drug effects Chlorides / toxicity Glutathione / analysis Neurons / drug effects* Orotic Acid / toxicity PC12 Cells Rats Zinc Acetate / toxicity Zinc Compounds / toxicity* Zinc Sulfate / toxicity |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Zinc Compounds; 56-65-5/Adenosine Triphosphate; 5970-45-6/Zinc Acetate; 65-86-1/Orotic Acid; 70-18-8/Glutathione; 7646-85-7/zinc chloride; 7733-02-0/Zinc Sulfate; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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