Document Detail


Comparative immunotoxicology of ultraviolet B exposure I. Effects of in vitro and in situ ultraviolet B exposure on the functional activity and morphology of Langerhans cells in the skin of different species.
MedLine Citation:
PMID:  9767236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ultraviolet (UV) B-induced morphological and functional changes in the skin of mice, rats and humans were investigated. Changes in the morphological structure of Langerhans cells (LC), the major antigen-presenting cells in the skin, using confocal laser scanning microscopy, were found in mouse and rat skin after in situ exposure to high doses of UVB radiation (FS40) (3-9 kJ/m2). Similar UVB doses failed to induce alterations in the morphological structure of human LC. Alterations in the function of epidermal cells (especially LC) were studied, using the mixed skin lymphocyte response (MSLR). In vitro UVB exposure of epidermal cells (EC), derived from the skin of the different species, revealed that low doses of UVB radiation impaired the stimulatory capacity of these cells dose-dependently; mouse epidermal cells were most UVB-susceptible, while human cells were least UVB susceptible. For suppression of the stimulatory capacity of EC after in situ UVB exposure of skin tissue, higher doses of UVB radiation than the in vitro UVB exposure were needed in all species tested. Also in this in situ set-up mouse epidermal cells were most UVB-susceptible, and human epidermal cells were least UVB-susceptible. The magnitude of differences in susceptibility for UVB-induced changes in the stimulatory capacity of EC after in situ and after in vitro exposure experiments was similar. Firstly, it may be concluded that UVB impairs the functional activity of LC at a lower dose than that which alters the morphology of these cells. Secondly, it is clear that epidermal cells, especially LC, from the skin of rodents are more susceptible to UVB than epidermal cells derived from human skin. It is important to account for these differences in susceptibility when data on the effects of UVB radiation on the immune system in rodents are extrapolated to humans.
Authors:
W Goettsch; H M Hurks; J Garssen; A M Mommaas; W Slob; J Hoekman; F Pierik; P J Roholl; H Van Loveren
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of dermatology     Volume:  139     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1999-01-19     Completed Date:  1999-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  230-8     Citation Subset:  IM    
Affiliation:
National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dose-Response Relationship, Radiation
Epidermis / radiation effects
Humans
Immune Tolerance*
Langerhans Cells / cytology,  immunology,  radiation effects*
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred Strains
Microscopy, Confocal
Rats
Rats, Wistar
Skin / immunology,  radiation effects*
Species Specificity
Ultraviolet Rays*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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