Document Detail

Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans.
MedLine Citation:
PMID:  23194436     Owner:  NLM     Status:  MEDLINE    
It was postulated that the highly virulent JP2 genotype of Aggregatibacter actinomycetemcomitans may possess a constellation of distinct virulence determinants not found in non-JP2 genotypes. This study compared the genome content and the transcriptome of the serotype b JP2 genotype and the closely related serotype b non-JP2 genotype of A. actinomycetemcomitans. A custom-designed pan-genomic microarray of A. actinomycetemcomitans was constructed and validated against a panel of 11 sequenced reference strains. The microarray was subsequently used for comparative genomic hybridization of serotype b strains of JP2 (six strains) and non-JP2 (six strains) genotypes, and for transcriptome analysis of strains of JP2 (three strains) and non-JP2 (two strains). Two JP2-specific and two non-JP2-specific genomic islands were identified. In one instance, distinct genomic islands were found to be inserted into the same locus among strains of different genotypes. Transcriptome analysis identified five operons, including the leukotoxin operon, to have at least two genes with an expression ratio of 2 or greater between genotypes. Two of the differentially expressed operons were members of the membrane-bound nitrate reductase system (nap operon) and the Tol-Pal system of gram-negative bacterial species. This study is the first to demonstrate the differences in the full genome content and gene expression between A. actinomycetemcomitans strains of JP2 and non-JP2 genotypes. The information is essential for designing hypothesis-driven experiments to examine the pathogenic mechanisms of A. actinomycetemcomitans.
Y Huang; W Kittichotirat; M P A Mayer; R Hall; R Bumgarner; C Chen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-10-12
Journal Detail:
Title:  Molecular oral microbiology     Volume:  28     ISSN:  2041-1014     ISO Abbreviation:  Mol Oral Microbiol     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-11     Completed Date:  2013-12-11     Revised Date:  2014-02-17    
Medline Journal Info:
Nlm Unique ID:  101524770     Medline TA:  Mol Oral Microbiol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1-17     Citation Subset:  D    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Aggregatibacter actinomycetemcomitans / genetics*,  pathogenicity
Bacterial Outer Membrane Proteins / genetics
Bacterial Toxins / genetics
Chromosome Mapping
Comparative Genomic Hybridization / methods*
Exotoxins / genetics
Gene Expression Profiling / methods*
Gene Expression Regulation, Bacterial / genetics
Genome, Bacterial / genetics*
Genomic Islands / genetics
Microarray Analysis / methods*
Middle Aged
Multigene Family / genetics
Nitrate Reductase / genetics
Operon / genetics
Promoter Regions, Genetic / genetics
Sensitivity and Specificity
Transcriptome / genetics
Virulence / genetics
Young Adult
Grant Support
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Toxins; 0/Exotoxins; 0/leukotoxin; EC Reductase

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