| Comparative genomic analysis reveals species-dependent complexities that explain difficulties with microsatellite marker development in molluscs. | |
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MedLine Citation:
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PMID: 20424639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reliable population DNA molecular markers are difficult to develop for molluscs, the reasons for which are largely unknown. Identical protocols for microsatellite marker development were implemented in three gastropods. Success rates were lower for Gibbula cineraria compared to Littorina littorea and L. saxatilis. Comparative genomic analysis of 47.2 kb of microsatellite containing sequences (MCS) revealed a high incidence of cryptic repetitive DNA in their flanking regions. The majority of these were novel, and could be grouped into DNA families based upon sequence similarities. Significant inter-specific variation in abundance of cryptic repetitive DNA and DNA families was observed. Repbase scans show that a large proportion of cryptic repetitive DNA was identified as transposable elements (TEs). We argue that a large number of TEs and their transpositional activity may be linked to differential rates of DNA multiplication and recombination. This is likely to be an important factor explaining inter-specific variation in genome stability and hence microsatellite marker development success rates. Gastropods also differed significantly in the type of TEs classes (autonomous vs non-autonomous) observed. We propose that dissimilar transpositional mechanisms differentiate the TE classes in terms of their propensity for transposition, fixation and/or silencing. Consequently, the phylogenetic conservation of non-autonomous TEs, such as CvA, suggests that dispersal of these elements may have behaved as microsatellite-inducing elements. Results seem to indicate that, compared to autonomous, non-autonomous TEs maybe have a more active role in genome rearrangement processes. The implications of the findings for genomic rearrangement, stability and marker development are discussed. |
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Authors:
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C E McInerney; A L Allcock; M P Johnson; D A Bailie; P A Prodöhl |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-28 |
Journal Detail:
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Title: Heredity Volume: 106 ISSN: 1365-2540 ISO Abbreviation: Heredity (Edinb) Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-15 Completed Date: 2011-03-17 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 0373007 Medline TA: Heredity (Edinb) Country: England |
Other Details:
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Languages: eng Pagination: 78-87 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, Belfast, Northern Ireland, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Comparative Genomic Hybridization Gastropoda / genetics*, growth & development* Genetic Markers / genetics Genetic Variation* Microsatellite Repeats / genetics* Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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