Document Detail


Comparative gene expression profiles of intestinal transporters in mice, rats and humans.
MedLine Citation:
PMID:  17681807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied gene expression profiles of intestinal transporters in model animals and humans. Total RNA was isolated from duodenum and the mRNA expression was measured using Affymetrix GeneChip oligonucleotide arrays. Detected genes from the intestine of mice, rats, and humans were about 60% of 22,690 sequences, 40% of 8739, and 47% of 12,559, respectively. A total of 86 genes involving transporters expressed in mice, 50 genes in rats, and 61 genes in humans were detected. Mice exhibited abundant mRNA expressions for peptide transporter HPT1, amino acid transporters CSNU3, CT1 and ASC1, nucleoside transporter CNT2, organic cation transporter SFXN1, organic anion transporter NBC3, glucose transporter SGLT1, and fatty acid transporters FABP1 and FABP2. Rats showed high expression profiles of peptide transporter PEPT1, amino acid transporters CSNU1 and 4F2HC, nucleoside transporter CNT2, organic cation transporter OCT5, organic anion transporter SDCT1, glucose transporter GLUT2 and GLUT5, and folate carrier FOLT. In humans, the highly expressed genes were peptide transporter HPT1, amino acid transporters LAT3, 4F2HC and PROT, nucleoside transporter CNT2, organic cation transporter OCTN2, organic anion transporters NADC1, NBC1 and SBC2, glucose transporters SGLT1 and GLUT5, multidrug resistance-associated protein RHO12, fatty acid transporters FABP1 and FABP2, and phosphate carrier PHC. Overall these data reveal diverse transcriptomic profiles for intestinal transporters among these species. Therefore, this transcriptional data may lead to more effective use of the laboratory animals as a model for oral drug development.
Authors:
Hye-Ryoung Kim; Sung-Won Park; Hee-Jung Cho; Kyung-Ae Chae; Ji-Min Sung; Jin-Suk Kim; Christopher P Landowski; Duxin Sun; A M Abd El-Aty; Gordon L Amidon; Ho-Chul Shin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-23
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  56     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-03     Completed Date:  2007-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  224-36     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Transport Systems / analysis
Animals
Carrier Proteins / analysis*,  genetics,  metabolism
Duodenum / chemistry*,  metabolism
Gene Expression*
Gene Expression Profiling / methods
Glucose Transport Proteins, Facilitative / analysis
Humans
Mice
Multidrug Resistance-Associated Proteins / analysis
Nucleoside Transport Proteins / analysis
Oligonucleotide Array Sequence Analysis
Organic Anion Transporters / analysis
Organic Cation Transport Proteins / analysis
P-Glycoproteins / analysis
Peptides / metabolism
RNA, Messenger / analysis*
Rats
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Carrier Proteins; 0/Glucose Transport Proteins, Facilitative; 0/Multidrug Resistance-Associated Proteins; 0/Nucleoside Transport Proteins; 0/Organic Anion Transporters; 0/Organic Cation Transport Proteins; 0/P-Glycoproteins; 0/Peptides; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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