Document Detail

Comparative fluorescence spectroscopy of root caries lesions.
MedLine Citation:
PMID:  15560831     Owner:  NLM     Status:  MEDLINE    
In this study, the light-emission properties of carious and sound root surfaces were investigated under a wide range of excitation wavelengths. Human molar teeth with exposed root surfaces containing light- and dark-discolored root caries (n = 3 of each) were selected. Emission spectra were recorded from carious and corresponding sound root surface areas from each tooth by using a fluorescence spectrophotometer at excitation wavelengths from 360 nm up to 580 nm, in steps of 20 nm. The spectra were corrected for fluctuations in detector sensitivity and excitation light intensity, and normalized to peak intensity. Excitation spectra were recorded for selected emission wavelengths that showed maximum intensity. Light- and dark-discolored root surface caries showed distinct fluorescence emission bands between 600 and 700 nm that were not present in sound root surface areas. These bands were strongest for excitation wavelengths between 390 and 420 nm. The excitation spectra of root caries revealed maximum excitation at around 405 nm, which is equivalent to the Soret band of porphyrin compounds. The emission spectra of both types of root caries lesions were shifted towards longer wavelengths (red shift at half maximum) when compared to the spectra of corresponding sound root surfaces. The red shift for dark-discolored root caries was higher than for light-discolored lesions at all excitation wavelengths.
Wolfgang Buchalla; Aine M Lennon; Thomas Attin
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of oral sciences     Volume:  112     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-24     Completed Date:  2005-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  490-6     Citation Subset:  D; IM    
Copyright Information:
Copyright Eur J Oral Sci, 2004.
Department of Operative Dentistry, Preventive Dentistry and Periodontology, Georg August University, Robert-Koch-Str. 40, D-37075 Göttingen, Germany.
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MeSH Terms
Dentin / physiopathology
Molar / physiopathology
Porphyrins / analysis
Root Caries / physiopathology*
Spectrometry, Fluorescence / instrumentation,  methods
Tooth Discoloration / physiopathology
Tooth Root / physiopathology
Reg. No./Substance:

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