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Comparative expression of hCG beta genes in human trophoblast from early and late first-trimester placentas.
MedLine Citation:
PMID:  22811468     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Human chorionic gonadotropin (hCG) displays a major role in pregnancy initiation and progression and is involved in trophoblast differentiation and fusion. However, the site and the type of dimeric hCG production during the first trimester of pregnancy is poorly known. At that time trophoblastic plugs present in the uterine arteries disappear, allowing unrestricted flow of maternal blood to the intervillous space. The consequence is an important modification of the trophoblast environment including a rise of oxygen levels from about 2.5% prior to 10 weeks of amenorrhea (WA) to approximately 8% after 12 WA. Two specific βhCG proteins that differ from three amino acids have been described: type 1 (CGB7) and type 2 (CGB3, 5, 8). Here, we demonstrated in situ and ex vivo on placental villi and in vitro in primary cultures of human cytotrophoblasts that type 1 and 2 ßhCG RNAs and proteins were expressed by trophoblasts and that these expressions were higher before blood enters in the intervillous space (8-9 WA vs 12-14 WA). hCG was immunodetected in villous mononucleated cytotrophoblasts (VCT) and syncytiotrophoblast (ST) at 8-9 WA but only in ST at 12-14 WA. Furthermore, hCG secretion was 4-fold higher in VCT cultures from 8-9 WA compared to 12-14 WA. Interestingly, VCT from 8-9 WA placentas were found to exhibit more fusion features. Taken together, we showed that type 1 and type 2 ßhCG are highly expressed by VCT in early first trimester contributing to the high levels of hCG found in maternal serum at this term.
Authors:
Mélanie Cocquebert; Sarah Berndt; Nadine Segond; Jean Guibourdenche; Padma Murthi; Lydia Aldaz-Carroll; Danièle Evain-Brion; Thierry Fournier
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-17
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  -     ISSN:  1522-1555     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1INSERM.
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