Document Detail

Comparative expression of hCG beta genes in human trophoblast from early and late first-trimester placentas.
MedLine Citation:
PMID:  22811468     Owner:  NLM     Status:  Publisher    
Human chorionic gonadotropin (hCG) displays a major role in pregnancy initiation and progression and is involved in trophoblast differentiation and fusion. However, the site and the type of dimeric hCG production during the first trimester of pregnancy is poorly known. At that time trophoblastic plugs present in the uterine arteries disappear, allowing unrestricted flow of maternal blood to the intervillous space. The consequence is an important modification of the trophoblast environment including a rise of oxygen levels from about 2.5% prior to 10 weeks of amenorrhea (WA) to approximately 8% after 12 WA. Two specific βhCG proteins that differ from three amino acids have been described: type 1 (CGB7) and type 2 (CGB3, 5, 8). Here, we demonstrated in situ and ex vivo on placental villi and in vitro in primary cultures of human cytotrophoblasts that type 1 and 2 ßhCG RNAs and proteins were expressed by trophoblasts and that these expressions were higher before blood enters in the intervillous space (8-9 WA vs 12-14 WA). hCG was immunodetected in villous mononucleated cytotrophoblasts (VCT) and syncytiotrophoblast (ST) at 8-9 WA but only in ST at 12-14 WA. Furthermore, hCG secretion was 4-fold higher in VCT cultures from 8-9 WA compared to 12-14 WA. Interestingly, VCT from 8-9 WA placentas were found to exhibit more fusion features. Taken together, we showed that type 1 and type 2 ßhCG are highly expressed by VCT in early first trimester contributing to the high levels of hCG found in maternal serum at this term.
Mélanie Cocquebert; Sarah Berndt; Nadine Segond; Jean Guibourdenche; Padma Murthi; Lydia Aldaz-Carroll; Danièle Evain-Brion; Thierry Fournier
Related Documents :
10571768 - Repeated adverse fetal outcome in pregnancy complicated by uncontrolled maternal phenyl...
8065668 - The association of maternal social class with maternal diet and the dimensions of babie...
19966268 - Maternal metabolism and obesity: modifiable determinants of pregnancy outcome.
12802378 - Effects of maternal oxygen supplementation on fetal oxygenation and lipid peroxidation ...
2218788 - Symptomatic rubella re-infection in early pregnancy and subsequent delivery of an infec...
16885668 - Vaginal misoprostol administration for cervical ripening and labor induction.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-17
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  -     ISSN:  1522-1555     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular inflammation and adipose tissue matrix remodeling precede physiological adaptations to pre...
Next Document:  Sertoli-secreted FGF-2 induces PFKFB4 isozyme expression in mouse spermatogenic cells by activation ...