| Comparative evaluation of target-specific GFP gene silencing efficiencies for antisense ODN, synthetic siRNA, and siRNA plasmid complexed with PEI-PEG-FOL conjugate. | |
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MedLine Citation:
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PMID: 16417275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell specific gene silencing effects of antisense oligodeoxynucleotide (AS-ODN), synthetic small interfering RNA (siRNA-S), and siRNA expressing plasmid (siRNA-P) were comparatively evaluated. Poly(ethylenimine) (PEI) and PEI-graft-poly(ethylene glycol)-folate (PEI-PEG-FOL) conjugate were used to form nanosized polyelectrolyte complexes with the above three nucleic acids coding for inhibition of green fluorescent protein (GFP) expression. The three nucleic acid complexes formulated with either PEI or PEI-PEG-FOL had comparable sizes and surface zeta potential values. Among the three inhibitory nucleic acids, siRNA-S, when complexed with PEI-PEG-FOL, exhibited the most dose-effective and fastest gene silencing effect for FOL receptor overexpressing KB cells, because the siRNA-S could be directly delivered, via FOL receptor-mediated endocytosis, into the cytoplasm compartment where the degradation processing of target GFP mRNA occurred in a sequence-specific manner. |
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Authors:
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Sun Hwa Kim; Hyejung Mok; Ji Hoon Jeong; Sung Wan Kim; Tae Gwan Park |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 17 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2006 Jan-Feb |
Date Detail:
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Created Date: 2006-01-18 Completed Date: 2006-09-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 241-4 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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metabolism Cell Line, Tumor Drug Carriers Folic Acid / administration & dosage, chemistry Gene Expression Gene Silencing / drug effects* Green Fluorescent Proteins / genetics*, metabolism Humans Oligodeoxyribonucleotides, Antisense / administration & dosage*, chemistry, metabolism Plasmids / genetics Polyethylene Glycols / administration & dosage, chemistry Polyethyleneimine / administration & dosage, chemistry RNA, Small Interfering / administration & dosage*, chemistry, metabolism Receptors, Cell Surface / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Drug Carriers; 0/Oligodeoxyribonucleotides, Antisense; 0/Polyethylene Glycols; 0/RNA, Small Interfering; 0/Receptors, Cell Surface; 0/folate-binding protein; 147336-22-9/Green Fluorescent Proteins; 59-30-3/Folic Acid; 9002-98-6/Polyethyleneimine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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