| Comparative evaluation of supplemented peptone broth with sodium polyanetholesulfonate and trypticase soy broth with sodium amylosulfate for detection of septicemia. | |
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MedLine Citation:
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PMID: 6286716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We compared the yield and speed of detection of clinically important microorganisms from 10,156 paired 5-ml samples of blood cultured in supplemented peptone broth (SPB) with 0.03% sodium polyanetholesulfonate (SPS) or Trypticase soy broth (TSB) with 0.5% sodium amylosulfate (SAS). The atmosphere of incubation (open venting units) and ratio of blood to broth (1:10) were the same for both samples. Only cultures with adequate blood samples (greater than or equal to 80% of stated volume) were compared statistically. Overall, SPB/SPS outperformed TSB/SAS. Bacteroidaceae and Eubacterium were found more often (P less than 0.05) and viridans streptococci were found sooner (P less than 10(-4)) in SPB/SPS than in TSB/SAS. Most importantly, staphylococci were found both more often (P less than 0.03) and sooner (P less than 10(-7)) in SPB/SPS than in TSB/SAS. In a separate experiment, SAS slowed the growth of a clinical strain of Staphylococcus aureus in TSB. Unless important advantages can be confirmed for SAS in controlled clinical trials, SAS cannot be recommended for routine use as an anticoagulant in blood culture media. |
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Authors:
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J H Tenney; L B Reller; W L Wang; R L Cox; S Mirrett |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 16 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-10-21 Completed Date: 1982-10-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 107-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amylopectin* Anticoagulants* Bacteroidaceae / growth & development, isolation & purification* Benzenesulfonates* Culture Media* Eubacterium / growth & development, isolation & purification* Humans Polyanetholesulfonate* Sepsis / diagnosis, microbiology* Staphylococcus aureus / growth & development, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Benzenesulfonates; 0/Culture Media; 55963-78-5/Polyanetholesulfonate; 9010-01-9/amylosulfate sodium; 9037-22-3/Amylopectin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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