| Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces. | |
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MedLine Citation:
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PMID: 8748268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three methods of isolating Cryptosporidium parvum oocysts from rat feces were evaluated. Oocysts were initially isolated by sucrose density gradient centrifugation. They were further purified by passage through a glass bead column or a Percoll gradient or by dialysis. Although oocysts recovered from the glass bead column and by dialysis were relatively free of fecal debris, only oocysts recovered from the Percoll gradient were free of bacteria. Recovered oocysts retained their antigenicity and infectivity. The ability to effectively recover oocysts from rat feces suggests that the laboratory rat may be a convenient substitute for ruminants in the propagation and maintenance of C. parvum oocysts for in vitro and in vivo use. |
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Authors:
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P Suresh; J E Rehg |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 34 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-10-11 Completed Date: 1996-10-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 38-40 Citation Subset: IM |
Affiliation:
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Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101, USA. suresh@mbcf.stjude.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Centrifugation, Density Gradient Cryptosporidium parvum / immunology, isolation & purification*, pathogenicity Dialysis Evaluation Studies as Topic Feces / parasitology* Female Glass Parasitology / methods* Povidone Rats Rats, Sprague-Dawley Silicon Dioxide |
| Grant Support | |
ID/Acronym/Agency:
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AI-33288/AI/NIAID NIH HHS; CA-21765/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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