Document Detail


Comparative evaluation of microultrafiltration devices for determination of protein binding.
MedLine Citation:
PMID:  3176109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The free fraction of drugs was determined comparatively with new microultrafiltration devices, Molcut II (Catalogue No. SJGC type) (MLII) and (Catalogue No. PLGC type) (NML; a device derived by changing the membrane component of MLII), which were developed by Nihon Millipore Ltd., Tokyo, Japan. The results obtained with the device are compared with those of MPS-1 (MPS), which is commercially available from Amicon Co., Danvers, MA, U.S.A. Drugs tested are phenytoin, carbamazepine, valproic acid, theophylline, phenobarbital, and gliclazide. In five of six drugs the free fraction by MLII shows lower values, although that by NML is in good agreement with MPS. The lower values of free fraction of drugs obtained with MLII may be caused by adsorption to the device. The adsorption increase is relatively correlated to increasing lipophilicity of drugs. The results suggest that physicochemical properties of each drug, especially hydrophobicity, should be taken into account, when a new device is used to examine protein binding of drugs. The NML, which is less expensive and is convenient because no special instrument is needed, is a reliable and satisfactory device for routine therapeutic free drug monitoring.
Authors:
T Ohshima; I Johno; S Kitazawa
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Therapeutic drug monitoring     Volume:  10     ISSN:  0163-4356     ISO Abbreviation:  Ther Drug Monit     Publication Date:  1988  
Date Detail:
Created Date:  1988-11-15     Completed Date:  1988-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909660     Medline TA:  Ther Drug Monit     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  310-5     Citation Subset:  IM    
Affiliation:
Department of Hospital Pharmacy, School of Medicine, Nagoya University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Blood Proteins / metabolism*
Humans
Pharmaceutical Preparations / blood
Protein Binding
Ultrafiltration / instrumentation*
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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