| Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates. | |
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MedLine Citation:
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PMID: 22850946 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The microplate nitrate reductase assay (MNRA) and the rezasurin microtitre assay (REMA) were used for the susceptibility testing of 73 clinical isolates and the results were compared with those that were obtained using the Bactec 460 TB and Bactec MGIT 960 systems. The REMA and the MNRA were performed in 96-well plates. For the REMA, the concentrations of isoniazid (INH) and rifampicin (RIF) ranged from 1.0-0.01 µg/mL and 2.0-0.03 µg/mL, respectively. For the MNRA, the INH concentration was between 1.0-0.03 µg/mL and the RIF concentration was between 2.0-0.06 µg/mL. For the MNRA, the sensitivity, specificity, positive predictive value, negative predictive value and INH/RIF agreement were 100/95.6, 97.6/100, 96.8/100, 100/98 and 98.6/98.6, respectively, and for the REMA, they were 100/91.3, 90.4/100, 88.5/100, 100/96.1 and 94.5/97.2, respectively. Our data suggest that these two rapid, low-cost methods may be inexpensive, alternative assays for the rapid detection of multidrug resistant tuberculosis in low-income countries. |
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Authors:
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Ahmet Yilmaz Coban; Meltem Uzun; Alper Akgunes; Belma Durupinar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Memórias do Instituto Oswaldo Cruz Volume: 107 ISSN: 1678-8060 ISO Abbreviation: Mem. Inst. Oswaldo Cruz Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502619 Medline TA: Mem Inst Oswaldo Cruz Country: Brazil |
Other Details:
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Languages: eng Pagination: 578-81 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Medical School, Ondokuz Mayis University, Samsun, Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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