| Comparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis. | |
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MedLine Citation:
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PMID: 18160087 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five serological tests for the diagnosis of visceral leishmaniasis (VL) were compared: a direct agglutination test (DAT) based on freeze-dried antigen (DAT-fd); a locally produced DAT (DAT-LPC); an IgG ELISA against rK39 (ELISA-rK39); an IgG ELISA for Leishmania chagasi (ELISA-L. chagasi); and an IgG IFAT against L. chagasi. Serum samples from 88 patients with VL, 20 non-infected individuals and 85 patients with others infectious diseases were evaluated. The sensitivity rates were: DAT-fd, 96.6%; DAT-LPC, 95.5%; ELISA-rK39, 88.6%; ELISA-L. chagasi, 89.8%; and IFAT, 92.0% (P>0.05). The specificity for the control groups varied from 53.3% to 100%. DAT-fd had the highest efficiency (97.4%), followed by DAT-LPC (91.7%) and ELISA-rK39 (90.7%). Our data suggest that DAT-fd, DAT-LPC and ELISA-rK39 are useful tests for the diagnosis of VL and could replace IFAT as the routine diagnostic test in Brazil. |
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Authors:
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Mariana Junqueira Pedras; Luciana de Gouvêa Viana; Edward José de Oliveira; Ana Rabello |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-26 |
Journal Detail:
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Title: Transactions of the Royal Society of Tropical Medicine and Hygiene Volume: 102 ISSN: 0035-9203 ISO Abbreviation: Trans. R. Soc. Trop. Med. Hyg. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-14 Completed Date: 2008-05-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506129 Medline TA: Trans R Soc Trop Med Hyg Country: England |
Other Details:
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Languages: eng Pagination: 172-8 Citation Subset: IM |
Affiliation:
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Laboratory of Clinical Research, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenue Augusto de Lima, 1715, 30190002 Belo Horizonte, MG, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Agglutination Tests / methods Animals Antibodies, Protozoan / blood* Antigens, Protozoan / immunology* Brazil / epidemiology Child Child, Preschool Endemic Diseases* Enzyme-Linked Immunosorbent Assay / methods Female Fluorescent Antibody Technique, Indirect / methods Humans Immunologic Tests / methods*, standards Infant Leishmania / immunology* Leishmaniasis, Visceral / diagnosis* Male Middle Aged Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/Antigens, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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