Document Detail

Comparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis.
MedLine Citation:
PMID:  18160087     Owner:  NLM     Status:  MEDLINE    
Five serological tests for the diagnosis of visceral leishmaniasis (VL) were compared: a direct agglutination test (DAT) based on freeze-dried antigen (DAT-fd); a locally produced DAT (DAT-LPC); an IgG ELISA against rK39 (ELISA-rK39); an IgG ELISA for Leishmania chagasi (ELISA-L. chagasi); and an IgG IFAT against L. chagasi. Serum samples from 88 patients with VL, 20 non-infected individuals and 85 patients with others infectious diseases were evaluated. The sensitivity rates were: DAT-fd, 96.6%; DAT-LPC, 95.5%; ELISA-rK39, 88.6%; ELISA-L. chagasi, 89.8%; and IFAT, 92.0% (P>0.05). The specificity for the control groups varied from 53.3% to 100%. DAT-fd had the highest efficiency (97.4%), followed by DAT-LPC (91.7%) and ELISA-rK39 (90.7%). Our data suggest that DAT-fd, DAT-LPC and ELISA-rK39 are useful tests for the diagnosis of VL and could replace IFAT as the routine diagnostic test in Brazil.
Mariana Junqueira Pedras; Luciana de Gouvêa Viana; Edward José de Oliveira; Ana Rabello
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-26
Journal Detail:
Title:  Transactions of the Royal Society of Tropical Medicine and Hygiene     Volume:  102     ISSN:  0035-9203     ISO Abbreviation:  Trans. R. Soc. Trop. Med. Hyg.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506129     Medline TA:  Trans R Soc Trop Med Hyg     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-8     Citation Subset:  IM    
Laboratory of Clinical Research, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenue Augusto de Lima, 1715, 30190002 Belo Horizonte, MG, Brazil.
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MeSH Terms
Agglutination Tests / methods
Antibodies, Protozoan / blood*
Antigens, Protozoan / immunology*
Brazil / epidemiology
Child, Preschool
Endemic Diseases*
Enzyme-Linked Immunosorbent Assay / methods
Fluorescent Antibody Technique, Indirect / methods
Immunologic Tests / methods*,  standards
Leishmania / immunology*
Leishmaniasis, Visceral / diagnosis*
Middle Aged
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Antigens, Protozoan

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