Document Detail


Comparative effects of UW and SLS solutions on concentrative proline uptake in cold preserved rat hepatocytes.
MedLine Citation:
PMID:  10667097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous studies, we have shown that the rate of cell swelling induced by concentrative proline uptake in isolated rat hepatocytes decreased by 50 per cent after only 24 h of cold storage in University of Wisconsin (UW) solution, thereby representing a sensitive marker of alterations in hepatocyte functions after cold preservation and rewarming. We have thus used concentrative proline uptake to compare the capacity of UW and sodium-lactobionate-sucrose (SLS) solutions to maintain such differentiated hepatocyte functions. Isolated rat hepatocytes were kept at 4 degrees C for 4, 10, 24 and 48 h in UW or SLS solutions, and subsequently cultured at 37 degrees C for 1-2 h. Viability was measured by Trypan blue exclusion. After rewarming, cells were subjected to a 10 min administration of 10 mM proline and accumulation of the amino acid was assessed by changes in cell volume as measured by digital analysis of single-cell images obtained under bright-field illumination. Cell viability was reduced gradually and significantly after 0 to 48 h of preservation, and rewarming amplified this effect. However, loss of viability was similar in UW- and SLS-stored cells, as were initial steady-state cell volumes. Proline-induced swelling rate was reduced significantly by 13, 46 and by 57 per cent after 10, 24 and 48 h of preservation in UW solution, respectively. There is no significant difference between SLS- and UW-preserved hepatocyte swelling rates after 10 h and 48 h of cold preservation. However, the decline in the swelling rate of SLS-preserved hepatocytes incubated for 24 h is significantly lower than that of their UW-preserved counterparts. These results show that the SLS solution can preserve differentiated hepatic functions as well as the UW solution does.
Authors:
H Serrar; L Musallam; P Haddad
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Thérapie     Volume:  54     ISSN:  0040-5957     ISO Abbreviation:  Therapie     Publication Date:    1999 Sep-Oct
Date Detail:
Created Date:  2000-03-16     Completed Date:  2000-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0420544     Medline TA:  Therapie     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  601-6     Citation Subset:  IM    
Affiliation:
Département de pharmacologie, Université de Montréal, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / pharmacology
Allopurinol / pharmacology
Animals
Cell Differentiation
Cell Size / drug effects
Cell Survival
Chlorpromazine / pharmacology
Cryopreservation / methods*
Disaccharides / pharmacology
Dose-Response Relationship, Drug
Glutathione / pharmacology
Insulin / pharmacology
Liver / cytology*
Liver Transplantation
Male
Organ Preservation Solutions*
Proline / metabolism*
Raffinose / pharmacology
Rats
Rats, Sprague-Dawley
Sucrose / pharmacology
Tissue Preservation / methods*
Chemical
Reg. No./Substance:
0/Disaccharides; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 0/sodium lactobionate sucrose solution; 11061-68-0/Insulin; 147-85-3/Proline; 315-30-0/Allopurinol; 50-53-3/Chlorpromazine; 512-69-6/Raffinose; 57-50-1/Sucrose; 58-61-7/Adenosine; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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