| Comparative effects of L-tryptophan and 1-methyl-tryptophan on immunoregulation induced by sperm, human pre-implantation embryo and trophoblast supernatants. | |
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MedLine Citation:
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PMID: 14672333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: The hypothesis that indoleamine 2,3-dioxygenase (IDO) is necessary to regulate lymphocyte functions at the feto-maternal interface has been postulated, although a possible role of tryptophan (Trp) depletion in the T-cell tolerance during insemination as well as implantation has not been previously investigated. METHOD OF STUDY: Allogeneic phytohaemagglutinin stimulated lymphocytes were supplemented with pre-implantation embryo supernatant (PES), seminal plasma (SP), spermatozoa culture supernatant (SCS), spermatozoa, trophoblast cells, or placenta explant culture supernatants, and analyzed for expression of CD25, CD71, and CD69. Trp-degrading activity was assessed by addition of 1-methyl-Tryptophan or L-Trp. RESULTS: PES, SP, trophoblast, and explant supernatants reduced the expression of CD25 in CD3 lymphocytes. Inhibition of IDO as well as Trp supplementation prevented these effects. CONCLUSIONS: These data suggest that the expression of interleukin-2 (IL-2) receptor in maternal T lymphocytes is normally suppressed by Trp catabolism, and that either abnormal IDO levels or substances influencing IDO activity might lead to non-adequate immune responses on sperm, harm the conceptus or even initiate fetal rejection. |
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Authors:
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G Gutiérrez; J S Fitzgerald; T Pöhlmann; I Hoppe; U R Markert |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 50 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 309-15 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Friedrich-Schiller-University, Jena, Germany. gabgutie@ffyb.uba.ar |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blastocyst / immunology* Female Humans Immune Tolerance / drug effects* Indoleamine-Pyrrole 2,3,-Dioxygenase Male Maternal-Fetal Exchange / immunology Pregnancy Receptors, Interleukin-2 / metabolism Spermatozoa / immunology* T-Lymphocytes / drug effects, immunology, metabolism Trophoblasts / immunology* Tryptophan / analogs & derivatives*, pharmacology* Tryptophan Oxygenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/1-methyl-tryptophan; 0/Receptors, Interleukin-2; 73-22-3/Tryptophan; EC 1.13.11.11/Tryptophan Oxygenase; EC 1.13.11.42/Indoleamine-Pyrrole 2,3,-Dioxygenase |
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