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Comparative development and tissue tropism in Nosema apis and Nosema ceranae.
MedLine Citation:
PMID:  23321524     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The two etiological agents of nosema disease in honey bees, Nosema apis and Nosema ceranae (Microsporidia: Nosematidae), reproduce in the midgut tissues of the host. Nosema apis is tissue specific but the development and tissue tropism of Nosema ceranae is not well understood. Our investigations compared development of the two phylogenetically related pathogens in all major host tissues. Using microscopy, PCR and qPCR quantification to evaluate tissue tropism of infected bees in communal cages and of individually restrained infected bees, we found no detectable spores in cephalic or other body tissues except midgut tissues. Nosema DNA was detected in Malpighian tubules but the tubules could not be separated from the alimentary tract without release of spores from the midgut. Nosema DNA was not detected in hemolymph sampled from the head capsule or the abdomen of infected bees. We confirmed that N. ceranae only develops in midgut tissues. Spores of both species released from host midgut cells accumulated in the hindgut lumen, and we noted differences in numbers and ratios of spore types and in growth curves between the two pathogens. Nosema apis reached a consistent level of spore production after 12 days post inoculation (dpi); Nosema ceranae spore production increased linearly from 12-20 dpi and the number of mature Nosema ceranae spores was consistently higher.
Authors:
Wei-Fone Huang; Leellen F Solter
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-12
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  -     ISSN:  1096-0805     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Inc.
Affiliation:
Illinois Natural History Survey, Prairie Research Institute, University of Illinois 1816 S. Oak Street, Champaign, IL 61801 USA. Electronic address: wfhuang@illinois.edu.
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