Document Detail

Comparative cytotoxicity of purified Shiga-like toxin-IIe on porcine and bovine aortic endothelial and human colonic adenocarcinoma cells.
MedLine Citation:
PMID:  8918947     Owner:  NLM     Status:  MEDLINE    
Porcine and bovine aortic endothelial cells and human colonic adenocarcinoma cells were compared for their susceptibility to the toxic effect of purified Shiga-like toxin IIe (SLT-IIe), measured by the neutral red cytotoxicity assay. Cytotoxicity correlated with toxin binding as indicated by fluorescence activated cell sorter analysis and with the globotriosylceramide (Gb3) and globotetraosylceramide (Gb4) content of cells determined by high pressure liquid chromatography. One line of porcine aortic endothelial cells was 1400-fold more susceptible than the line of bovine aortic endothelial cells that was tested, but a second line of porcine aortic endothelial cells was highly refractory to SLT-IIe. Human colonic adenocarcinoma cells lacked detectable levels of Gb4 and were least susceptible to SLT-IIe.
A Valdivieso-Garcia; D L MacLeod; R C Clarke; C L Gyles; C Lingwood; B Boyd; A Durette
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  45     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-12     Completed Date:  1996-12-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  331-7     Citation Subset:  IM    
Health of Animals Laboratory, Agriculture and Agri-Food Canada, Guelph, Ontario.
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MeSH Terms
Bacterial Toxins / toxicity*
Cell Line
Cell Survival / drug effects
Cells, Cultured
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Colonic Neoplasms
Cytotoxins / toxicity*
Endothelium, Vascular / drug effects*
Escherichia coli
Flow Cytometry
Globosides / analysis
Receptors, Cell Surface / analysis
Shiga Toxin 2
Trihexosylceramides / analysis
Tumor Cells, Cultured
Vero Cells
Reg. No./Substance:
0/Bacterial Toxins; 0/Cytotoxins; 0/Globosides; 0/Receptors, Cell Surface; 0/Shiga Toxin 2; 0/Trihexosylceramides; 11034-93-8/globotetraosylceramide; 71965-57-6/globotriaosylceramide

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