| Comparative chronic in vitro toxicity of hexavalent chromium to osteoblasts and monocytes. | |
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MedLine Citation:
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PMID: 18306314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is increasing concern regarding the long-term biological effects of exposure to low concentrations of metal ions, particularly chromium, in patients following implantation of metal-on-metal hip prostheses. To investigate this, we have evaluated the chronic in vitro response to clinically relevant concentrations of chromium VI in osteoblasts and monocytes over a 4-week period in continuous culture. The cell viability, intracellular reduced glutathione content, glutathione reductase activity and expression, and expression of glutamate cysteine ligase were monitored in both cell types. Monocytes were more susceptible to the toxicity of the metal, and at the end of 4 weeks exposure to 0.5 microM Cr VI, the protein content of cultures had declined to 23.4% +/- 1.0% of control cultures. Both cell types demonstrated temporal increases in reduced glutathione levels, glutathione reductase activity, and glutamate cysteine ligase expression. Only osteoblasts showed a transcriptional increase in reductase expression. Data suggest that both cell types mount an adaptive response to chronic exposure to Cr VI, but this is more potent in osteoblasts, and results in the relative resilience of this cell type to the effects of Cr VI on cell viability. |
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Authors:
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Vijay Krishna Raghunathan; Justice N A Tettey; Elizabeth M Ellis; M Helen Grant |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 88 ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2008-12-22 Completed Date: 2009-02-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 543-50 Citation Subset: IM |
Affiliation:
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Bioengineering Unit, Wolfson Centre, University of Strathclyde, Glasgow, Scotland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinogens, Environmental / toxicity* Cells, Cultured Chromium / toxicity* Glutathione / metabolism Glutathione Reductase / metabolism Hip Prosthesis / adverse effects Humans Middle Aged Monocytes / drug effects* Osteoblasts / drug effects* Prosthesis Failure Rats |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens, Environmental; 18540-29-9/chromium hexavalent ion; 70-18-8/Glutathione; 7440-47-3/Chromium; EC 1.8.1.7/Glutathione Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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