| Comparative characterization of glutamate decarboxylase in crude homogenates of oviduct, ovary, and hypothalamus. | |
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MedLine Citation:
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PMID: 6149264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Some biochemical characteristics of L-glutamate decarboxylase (GAD) were compared using crude homogenates of the rat oviduct, ovary, and hypothalamus. As estimated by the measurement of CO2 production, the specific activities of oviductal and ovarian GAD were about 10 and 3% of the hypothalamic value, respectively. Stoichiometric formation of gamma-aminobutyric acid (GABA) and CO2 from L-glutamate could be observed in oviduct and hypothalamus, while in ovarian homogenates the production of CO2 was more than nine times that of GABA. Hypothalamic and tubal GAD showed similar time course, temperature dependence, and pH dependence. The dependence on protein concentration and on exogenous cofactor supply was also similar in these two tissues. The kinetic constant for L-glutamate as a substrate was nearly the same in oviduct (1.30 mM) and hypothalamus (1.64 mM). The responsiveness of tubal and hypothalamic GAD to various inhibitors was also similar. The present findings indicate that the oviductal and the hypothalamic GAD may be identical, and they suggest a possible GABAergic innervation of the Fallopian tube. |
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Authors:
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S L Erdö; B Kiss; L Szporny |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of neurochemistry Volume: 43 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1984-12-20 Completed Date: 1984-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1532-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Dioxide / metabolism Fallopian Tubes / metabolism* Female Glutamate Decarboxylase / antagonists & inhibitors, metabolism* Glutamates / metabolism Glutamic Acid Hydrogen-Ion Concentration Hypothalamus / metabolism* Kinetics Ovary / metabolism* Pyridoxal Phosphate / pharmacology Rats Rats, Inbred Strains Temperature gamma-Aminobutyric Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glutamates; 124-38-9/Carbon Dioxide; 54-47-7/Pyridoxal Phosphate; 56-12-2/gamma-Aminobutyric Acid; 56-86-0/Glutamic Acid; EC 4.1.1.15/Glutamate Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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