Document Detail


Comparative challenge model of Flavobacterium columnare using abraded and unabraded channel catfish, Ictalurus punctatus (Rafinesque).
MedLine Citation:
PMID:  14513970     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The early entry of the fish pathogen Flavobacterium columnare and enhancement by abrasion was studied in channel catfish, Ictalurus punctatus (Rafinesque), using the polymerase chain reaction and a species-specific primer set for a bacterial 16S rRNA gene product. Evaluations were conducted following an abrasion bath immersion challenge with F. columnare. Abrasion, a practice which has historically been used prior to bacterial challenge, had significant effects on the early entry of the pathogen and on cumulative percent survival (CPS). The FvpF1-FvpR1 primer set was useful in detecting the early entry of F. columnare in mucus, skin, gill, blood, liver and trunk kidney tissues in both abraded and unabraded fish following immersion challenge at 29 +/- 2 degrees C. Bacteria were detected earlier in all tissues in abraded fish, except in the trunk kidney. These differences were not significant, except in the case of blood. Mucus, skin and gill tissues were positive for F. columnare earliest regardless of treatment (after 5 min in abraded fish and after 15 min in unabraded fish). CPS following challenge with F. columnare was significantly affected by abrasion, which supports the use of abrasion for the F. columnare challenge model for channel catfish.
Authors:
J A Bader; K E Nusbaum; C A Shoemaker
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of fish diseases     Volume:  26     ISSN:  0140-7775     ISO Abbreviation:  J. Fish Dis.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-29     Completed Date:  2003-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9881188     Medline TA:  J Fish Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-7     Citation Subset:  IM    
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Laboratory, Auburn, Alabama 36831-0952, USA. jbader@ars.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / genetics,  isolation & purification
Animals
Bacterial Proteins*
DNA / analysis
Fish Diseases / microbiology*
Flavobacterium* / genetics,  growth & development
Gram-Negative Bacterial Infections / veterinary*
Ictaluridae / injuries*,  microbiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 9007-49-2/DNA; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/protein-deamidating enzyme, Chryseobacterium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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