| Comparative capacities of the pig colon and duodenum for luminal iron absorption. | |
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MedLine Citation:
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PMID: 17487259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Iron deficiency is the most common human nutritional disorder in the world. Iron absorptive capacity of the small intestine is known to be much limited and therefore large quantities of iron salts must be used to treat iron deficiency. As a result, significant amounts of iron may reach the large intestine. This study compared the capacities of the small and large intestine to transfer luminal iron to the venous blood in relationship with the expression in epithelial cells of proteins involved in iron absorption using a pig model. Intracaecal injection of iron sulphate corresponding with 2.5 and 5.0 mg elemental iron per kg body mass resulted in modest, transient, but significant (p<0.05) increases in iron concentration in the portal blood plasma. By comparing portal blood plasma iron concentrations following injection in the duodenal and caecal lumen, we calculated that 5 h after injection, iron colonic absorption represented approximately 14% of duodenal absorption. Caecal and proximal colon mucosa accumulated iron to a much lower extent than the duodenal mucosa. Isolated colonocytes were found to express divalent metal transporter (DMT1) and ferritin, but to a lesser extent than the duodenal enterocytes. Ferroportin was highly expressed in colonocytes. In these cells as well as in enterocytes ferroportin was found to be glycosylated. In short term experiments and at a concentration in the range of that measured in the aqueous phases recovered from the large intestine luminal content after iron injection, iron sulphate did not alter colonocyte viability. We concluded that the colonic epithelial cells that express proteins involved in iron absorption are able to transfer luminal iron to the venous blood even if its relative participation in the overall intestinal absorption appears to be modest under our experimental conditions. |
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Authors:
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François Blachier; Pierre Vaugelade; Véronique Robert; Bertille Kibangou; François Canonne-Hergaux; Serge Delpal; François Bureau; Hervé Blottière; Dominique Bouglé |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 85 ISSN: 0008-4212 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-05-09 Completed Date: 2007-07-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: Canada |
Other Details:
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Languages: eng Pagination: 185-92 Citation Subset: IM |
Affiliation:
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Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France. Francois.Blachier@jouy.inra.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cation Transport Proteins / analysis Cell Survival Colon / cytology, metabolism* Duodenum / cytology, metabolism* Enterocytes / metabolism Ferritins / analysis Intestinal Absorption* Intestinal Mucosa / metabolism Iron / metabolism* Swine |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/metal transporting protein 1; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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