Document Detail

Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655.
MedLine Citation:
PMID:  11108008     Owner:  NLM     Status:  MEDLINE    
Two primer sets for direct sequence determination of all seven rRNA operons (rrn) of Escherichia coli have been developed; one is for specific-amplification of each rrn operon and the other is for direct sequencing of the amplified operons. Using these primer sets, we determined the nucleotide sequences of seven rrn operons, including promoter and terminator regions, of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai strain. To elucidate the intercistronic or intraspecific variation of rrn operons, their sequences were compared with those for the K-12 rrn operons. The rrn genes and the internal transcribed spacer regions showed a higher similarity to each other in each strain than between the corresponding operons of the two strains. However, the degree of intercistronic homogeneity was much higher in the EHEC strain than in K-12. In contrast, promoter and terminator regions in each operons were conserved between the corresponding operons of the two strains, which exceeded intercistronic similarity.
M Ohnishi; T Murata; K Nakayama; S Kuhara; M Hattori; K Kurokawa; T Yasunaga; K Yokoyama; K Makino; H Shinagawa; T Hayashi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  23     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-28     Completed Date:  2001-03-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  315-24     Citation Subset:  IM    
Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB035920;  AB035921;  AB035922;  AB035923;  AB035924;  AB035925
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MeSH Terms
Base Sequence
DNA Primers
DNA, Ribosomal Spacer
Escherichia coli / genetics*
Escherichia coli O157 / genetics*
Molecular Sequence Data
Operon / genetics*
Promoter Regions, Genetic
RNA, Ribosomal / genetics*
Regulatory Sequences, Nucleic Acid / genetics*
Terminator Regions, Genetic
Transcription, Genetic
Reg. No./Substance:
0/DNA Primers; 0/DNA, Ribosomal Spacer; 0/RNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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