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Comparative analysis of two methodologies for the identification of urinary red blood cell casts.
MedLine Citation:
PMID:  22189802     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
INTRODUCTION: Dysmorphic red blood cells (RBCs) in the urine are a strong indicator of a glomerular bleeding source. RBC casts, which while generally following RBC dysmorphism are not frequently seen on routine urinalysis, are also important indicators of glomerular hematuria. OBJECTIVE: This study tested the superiority of a urine concentration technique (CT) over the standard method (SM) for RBC cast identification in a group of patients suspected of glomerular hematuria. MATERIAL AND METHODS: Of a total of 4,227 routine urinary samples, 249 with dysmorphic hematuria were selected. The samples were processed according to two techniques: standard method (SM) and concentration technique (CT). The percentages of RBC cast identification according to each method were compared. RESULTS: The CT showed a higher rate of RBC casts (52.6%) compared to the SM (8.4%) (p < 0.001). DISCUSSION AND CONCLUSION: We suggest that the SM did not sufficiently concentrate the urine sample, the RBC casts remaining in the supernatant and being discarded. The CT increased the sensitivity of the RBC cast yield. The CT, associated with the presence of RBC dysmorphism, was useful to increase the agreement of the two parameters used for identification of glomerular-based bleeding and the diagnosis of glomerular diseases, important causes of chronic kidney disease.
Authors:
Carmen Antonia Sanches Ito; Roberto Pecoits-Filho; Larissa Bail; Mônica Arcoverde Wosiack; Danieli Afinovicz; Aline Borsato Hauser
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia     Volume:  33     ISSN:  2175-8239     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426946     Medline TA:  J Bras Nefrol     Country:  -    
Other Details:
Languages:  ENG; POR     Pagination:  402-407     Citation Subset:  -    
Affiliation:
Universidade Estadual de Ponta Grossa.
Vernacular Title:
Análise comparativa de duas metodologias para a identificação de cilindros hemáticos urinários.
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