| Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment. | |
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MedLine Citation:
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PMID: 18648817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Agrobacterium-mediated transformation and particle bombardment are the two most widely used methods for genetically modifying grasses. Here, these two systems are compared for transformation efficiency, transgene integration and transgene expression when used to transform tall fescue (Festuca arundinacea Schreb.). The bar gene was used as a selectable marker and selection during tissue culture was performed using 2 mg/l bialaphos in both callus induction and regeneration media. Average transformation efficiency across the four callus lines used in the experiments was 10.5% for Agrobacterium-mediated transformation and 11.5% for particle bombardment. Similar transgene integration patterns and co-integration frequencies of bar and uidA were observed in both gene transfer systems. However, while GUS activity was detected in leaves of 53% of the Agrobacterium transformed lines, only 20% of the bombarded lines showed GUS activity. Thus, Agrobacterium-mediated transformation appears to be the preferred method for producing transgenic tall fescue plants. |
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Authors:
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Caixia Gao; Danfeng Long; Ingo Lenk; Klaus Kristian Nielsen |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2008-07-22 |
Journal Detail:
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Title: Plant cell reports Volume: 27 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-15 Completed Date: 2009-03-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 1601-9 Citation Subset: IM |
Affiliation:
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Research Division, DLF-Trifolium Ltd, Hoejerupvej 31, 4660, Store Heddinge, Denmark. cxg@dlf.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biolistics* Blotting, Southern DNA, Plant / metabolism Festuca / genetics*, microbiology* Gene Dosage Gene Expression Regulation, Plant Genetic Vectors / genetics Plants, Genetically Modified Rhizobium / metabolism* Selection, Genetic Tissue Culture Techniques Transformation, Genetic* Transgenes |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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