Document Detail

Comparative analysis of transcriptional profiles between two apoptotic pathways of light-induced retinal degeneration.
MedLine Citation:
PMID:  15541899     Owner:  NLM     Status:  MEDLINE    
Light exposure can exacerbate the condition of a variety of human retinal diseases by increasing the rate of photoreceptor cell death. How light negatively affects photoreceptor cell survival is not yet fully understood. Previous studies involving light damage models have revealed two independent apoptotic pathways: low levels of light induce retinal degeneration in the arrestin -/- mouse via constitutive activation of the phototransduction cascade, whereas strong light exposure to the retina, such as in an albino eye, elicits photoreceptor cell death via activator protein (AP-1) induction. In order to better understand the initial gene expression changes underlying light damage, dark-reared arrestin -/- and albino BALB/c mice were exposed to constant white light (2000 lux), and their retinal morphology was assessed as a function of time. The expression profiles of retinal transcripts were then compared between dark-adapted and light-exposed arrestin -/-, pigmented wild-type and BALB/c mice at a time point when morphological changes were minimal. As expected, the dark-adapted samples showed little difference in expression pattern between the three genotypes. Among the genes differentially regulated by light in BALB/c, but not arrestin -/- retinas, were c-fos and other stress-induced early response genes. In both mouse models, a marked increase in expression of the bZIP family of transcription factors was observed. Our results show a select group of unique and overlapping sets of genes induced by light in the two mouse models. These expression changes may constitute the underlying initiating events leading to the two distinct mechanisms of light damage.
A Roca; K-J Shin; X Liu; M I Simon; J Chen
Related Documents :
1083249 - The rate of rhodopsin phosphorylation in isolated rentinas of frog and cattle.
7103819 - Choroidal blood flow ii. reflexive control in the monkey.
15589149 - Suppression of constant-light-induced blindness but not retinal degeneration by inhibit...
19729009 - Calpains are activated by light but their inhibition has no neuroprotective effect agai...
23375719 - Regional heart motion abnormality detection: an information theoretic approach.
6325009 - Ionic and spectral mechanisms of the off response to light in hyperpolarizing photorece...
2519589 - A circadian rhythm of population behavior in gonyaulax polyedra.
12122159 - Age-dependent variations in the directional sensitivity of balance corrections and comp...
10795629 - Flowers produce variations in color saturation by arranging petals at oblique and varyi...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience     Volume:  129     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2004  
Date Detail:
Created Date:  2004-11-15     Completed Date:  2005-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  779-90     Citation Subset:  IM    
Zilkha Neurogenetic Institute, The Mary D. Allen Laboratory for Vision Research, Beckman Macular Research Center, University of Southern California Keck School of Medicine, 1501 San Pablo Street, Los Angeles, CA 90033, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / physiology*,  radiation effects
Arrestin / deficiency,  genetics
Blotting, Western / methods
Dark Adaptation / physiology
In Situ Hybridization / methods
Light / adverse effects*
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Electron, Transmission / methods
Oligonucleotide Array Sequence Analysis / methods
Photoreceptor Cells, Vertebrate / pathology*,  ultrastructure
RNA, Messenger / biosynthesis
Reproducibility of Results
Retinal Degeneration / etiology*,  metabolism*,  pathology
Reverse Transcriptase Polymerase Chain Reaction / methods
Time Factors
Grant Support
Reg. No./Substance:
0/Arrestin; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  T lymphocytes play a role in neuropathic pain following peripheral nerve injury in rats.
Next Document:  Pre- and post-synaptic effects of manipulating surface charge with divalent cations at the photorece...