Document Detail


Comparative analysis of transcriptional and physiological responses of Bacillus cereus to organic and inorganic acid shocks.
MedLine Citation:
PMID:  19853945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Comparative phenotype and transcriptome analyses were performed with Bacillus cereus ATCC 14579 exposed to pH 5.5 set with different acidulants including hydrochloric acid (HCl), lactic acid (HL) and acetic acid (HAc). Phenotypes observed included a decreased growth rate (with HCl), bacteriostatic and bactericidal conditions, with 2mM undissociated HAc or HL, and 15mM undissociated HAc, respectively. In the latter condition a concomitant decrease in intracellular ATP levels was observed. The transcriptome analyses revealed general and specific responses to the acidulants used. The general acid stress response includes modulation of pyruvate metabolism with activation of the butanediol fermentation pathway, and an oxidative stress response that was, however, more extensive in the bacteriostatic and bactericidal conditions. HL-specific and HAc-specific responses include modulation of metabolic pathways for amino acid metabolism. Activation of lactate, formate, and ethanol fermentation pathways, alternative electron-transport chain components and fatty acid biosynthesis genes was noted in the presence of 15mM undissociated HAc. In conclusion, our study has provided insights in phenotype-associated, and general and acidulant-specific responses in B. cereus.
Authors:
Maarten Mols; Richard van Kranenburg; Marcel H Tempelaars; Willem van Schaik; Roy Moezelaar; Tjakko Abee
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-10-05
Journal Detail:
Title:  International journal of food microbiology     Volume:  137     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  13-21     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
Top Institute Food and Nutrition, Wageningen, The Netherlands; Laboratory of Food Microbiology, Wageningen University and Research Centre, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Acids
Adenosine Triphosphate / metabolism
Bacillus cereus / genetics*,  growth & development,  pathogenicity,  physiology*
Fermentation
Food Microbiology*
Gene Expression
Gene Expression Profiling
Genes, Bacterial
Hydrogen-Ion Concentration
Phenotype
Stress, Physiological
Chemical
Reg. No./Substance:
0/Acids; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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