| Comparative analysis of transcriptional and physiological responses of Bacillus cereus to organic and inorganic acid shocks. | |
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MedLine Citation:
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PMID: 19853945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Comparative phenotype and transcriptome analyses were performed with Bacillus cereus ATCC 14579 exposed to pH 5.5 set with different acidulants including hydrochloric acid (HCl), lactic acid (HL) and acetic acid (HAc). Phenotypes observed included a decreased growth rate (with HCl), bacteriostatic and bactericidal conditions, with 2mM undissociated HAc or HL, and 15mM undissociated HAc, respectively. In the latter condition a concomitant decrease in intracellular ATP levels was observed. The transcriptome analyses revealed general and specific responses to the acidulants used. The general acid stress response includes modulation of pyruvate metabolism with activation of the butanediol fermentation pathway, and an oxidative stress response that was, however, more extensive in the bacteriostatic and bactericidal conditions. HL-specific and HAc-specific responses include modulation of metabolic pathways for amino acid metabolism. Activation of lactate, formate, and ethanol fermentation pathways, alternative electron-transport chain components and fatty acid biosynthesis genes was noted in the presence of 15mM undissociated HAc. In conclusion, our study has provided insights in phenotype-associated, and general and acidulant-specific responses in B. cereus. |
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Authors:
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Maarten Mols; Richard van Kranenburg; Marcel H Tempelaars; Willem van Schaik; Roy Moezelaar; Tjakko Abee |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2009-10-05 |
Journal Detail:
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Title: International journal of food microbiology Volume: 137 ISSN: 1879-3460 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 13-21 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Top Institute Food and Nutrition, Wageningen, The Netherlands; Laboratory of Food Microbiology, Wageningen University and Research Centre, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acids Adenosine Triphosphate / metabolism Bacillus cereus / genetics*, growth & development, pathogenicity, physiology* Fermentation Food Microbiology* Gene Expression Gene Expression Profiling Genes, Bacterial Hydrogen-Ion Concentration Phenotype Stress, Physiological |
| Chemical | |
Reg. No./Substance:
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0/Acids; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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