| Comparative analysis of soluble organic matrix of axial skeleton and sclerites of Corallium rubrum: Insights for biomineralization. | |
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MedLine Citation:
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PMID: 21281736 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We analysed the soluble organic matrix (SOM) of two biominerals formed by the same organism but differing by their morphological characteristics: the axial skeleton and the sclerites of Corallium rubrum. Results of 1D SDS-PAGE electrophoresis show for the two biominerals that SOM proteins bands have similar apparent molecular weight but differ in quantity. Further analysis by 2D electrophoresis reveals each protein band as a line of spots with different isoelectric points. Our results suggest that each SOM protein band consists of a mix of proteins and/or one unique protein with post-translational modifications. By immunohistochemistry, we show that antibodies raised against SOM of axial skeleton and sclerites label the SOM of the two biominerals but also label insoluble organic matrix suggesting the presence of common epitopes between the two biominerals and the two organic fractions. |
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Authors:
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J Debreuil; S Tambutté; D Zoccola; N Segonds; N Techer; D Allemand; E Tambutté |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-28 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: - ISSN: 1879-1107 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier Inc. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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