| Comparative analysis of serum proteomes of patients with cardiovascular disease. | |
| | |
MedLine Citation:
|
PMID: 20934420 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVES: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. DESIGN AND METHODS: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. RESULTS: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P<0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. CONCLUSIONS: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients. |
| | |
Authors:
|
Hyun-Jung Kim; Hyun-Syuk Yoo; Pan-Kyeom Kim; Mi-Ryung Kim; Hyup-Woo Lee; Chan-Wha Kim |
Publication Detail:
|
Type: Journal Article Date: 2010-10-08 |
Journal Detail:
|
Title: Clinical biochemistry Volume: 44 ISSN: 1873-2933 ISO Abbreviation: Clin. Biochem. Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 178-84 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular markers and bladder carcinoma: Schistosomal and non-schistosomal.
Next Document: Direct and indirect requirements of Shh/Gli signaling in early pituitary development.