| Comparative analysis of minimal residual disease detection using four-color flow cytometry, consensus IgH-PCR, and quantitative IgH PCR in CLL after allogeneic and autologous stem cell transplantation. | |
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MedLine Citation:
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PMID: 15343348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The clinically most suitable method for minimal residual disease (MRD) detection in chronic lymphocytic leukemia is still controversial. We prospectively compared MRD assessment in 158 blood samples of 74 patients with CLL after stem cell transplantation (SCT) using four-color flow cytometry (MRD flow) in parallel with consensus IgH-PCR and ASO IgH real-time PCR (ASO IgH RQ-PCR). In 25 out of 106 samples (23.6%) with a polyclonal consensus IgH-PCR pattern, MRD flow still detected CLL cells, proving higher sensitivity of flow cytometry over PCR-genescanning with consensus IgH-primers. Of 92 samples, 14 (15.2%) analyzed in parallel by MRD flow and by ASO IgH RQ-PCR were negative by our flow cytometric assay but positive by PCR, thus demonstrating superior sensitivity of RQ-PCR with ASO primers. Quantitative MRD levels measured by both methods correlated well (r=0.93). MRD detection by flow and ASO IgH RQ-PCR were equally suitable to monitor MRD kinetics after allogeneic SCT, but the PCR method detected impending relapses after autologous SCT earlier. An analysis of factors that influence sensitivity and specificity of flow cytometry for MRD detection allowed to devise further improvements of this technique. |
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Authors:
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S Böttcher; M Ritgen; C Pott; M Brüggemann; T Raff; S Stilgenbauer; H Döhner; P Dreger; M Kneba |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leukemia Volume: 18 ISSN: 0887-6924 ISO Abbreviation: Leukemia Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-28 Completed Date: 2004-11-01 Revised Date: 2013-03-04 |
Medline Journal Info:
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Nlm Unique ID: 8704895 Medline TA: Leukemia Country: England |
Other Details:
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Languages: eng Pagination: 1637-45 Citation Subset: IM |
Affiliation:
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Second Department of Medicine, University of Kiel, Germany. s.boettcher@med2.uni-kiel.de <s.boettcher@med2.uni-kiel.de> |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Consensus Sequence Female Flow Cytometry / methods Humans Immunoglobulin Heavy Chains / genetics* Immunoglobulin Variable Region / genetics* Kinetics Leukemia, Lymphocytic, Chronic, B-Cell / genetics*, pathology, therapy Male Middle Aged Neoplasm, Residual / diagnosis* Polymerase Chain Reaction / methods Sensitivity and Specificity Stem Cell Transplantation* Transplantation, Autologous Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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