Document Detail

Comparative analysis of micro and macro B chromosomes in the Korean field mouse Apodemus peninsulae (Rodentia, Murinae) performed by chromosome microdissection and FISH.
MedLine Citation:
PMID:  15292605     Owner:  NLM     Status:  MEDLINE    
Comparative analysis of micro B and macro B chromosomes of the Korean field mouse Apodemus peninsulae, collected in populations from Siberia and the Russian Far East, was performed with Giemsa, DAPI, Ag-NOR staining and chromosome painting with whole and partial chromosome probes generated by microdissection and DOP-PCR. DNA composition of micro B chromosomes was different from that of macro B chromosomes. All analyzed micro B chromosomes contained clusters of DNA repeats associated with regions characterized by an uncondensed state in mitosis. Giemsa and DAPI staining did not reveal these regions. Their presence in micro B chromosomes led to their special morphology and underestimation in size. DNA repeat clusters homologous to DNA of micro B chromosome arms were also revealed in telomeric regions of some macro B chromosomes of specimens captured in Siberian regions. Neither active NORs nor clusters of ribosomal DNA were found in the uncondensed regions of micro B chromosomes. Possible evolutionary pathways for the origin of macro and micro B chromosomes are discussed.
N B Rubtsov; T V Karamysheva; O V Andreenkova; M N Bochkaerev; I V Kartavtseva; G V Roslik; Y M Borissov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  106     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2004  
Date Detail:
Created Date:  2004-08-04     Completed Date:  2005-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  289-94     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
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MeSH Terms
Bone Marrow Cells / cytology
Cells, Cultured
Chromosomes, Mammalian / chemistry*,  genetics*
DNA Probes / genetics
In Situ Hybridization, Fluorescence / methods*
Microdissection / methods*
Muridae / genetics*
X Chromosome / genetics
Y Chromosome / genetics
Reg. No./Substance:
0/DNA Probes

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