Document Detail

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses.
MedLine Citation:
PMID:  12810877     Owner:  NLM     Status:  MEDLINE    
The Rachiplusia ou multiple nucleopolyhedrovirus (RoMNPV) is a variant of Autographa californica MNPV (AcMNPV) but is significantly more virulent against several major agricultural pests. The genome sequence of the R1 strain of RoMNPV was determined and compared to that of AcMNPV strain C6. The RoMNPV genome is approximately 131.5 kbp with a G+C content of 39.1 %. The homologous repeat regions (hrs) described for AcMNPV-C6 are present in RoMNPV-R1 but the hrs of RoMNPV have fewer palindromic repeats. The RoMNPV-R1 nucleotide sequence is almost completely collinear with the sequence of AcMNPV-C6 and contains homologues of 150 of the 155 ORFs described for AcMNPV-C6. Deletions, insertions and substitutions have resulted in the loss of homologues for AcMNPV ORFs ac2 (bro), ac3 (ctl), ac97, ac121 and ac140 from the RoMNPV genome. The average amino acid sequence identity between RoMNPV and AcMNPV ORFs is 96.1 % and there are differences in promoter motif composition for 23 of these ORFs. Maximum-likelihood analysis of selection pressures on AcMNPV and RoMNPV ORFs indicate that ORFs ro18/ac20-ac21 (arif-1) and ro135/ac143 (odv-e18) have undergone positive selection.
Robert L Harrison; Bryony C Bonning
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  84     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-17     Completed Date:  2003-08-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1827-42     Citation Subset:  IM    
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA.
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MeSH Terms
Base Sequence
Cell Line
Cloning, Molecular
Consensus Sequence
Genome, Viral*
Molecular Sequence Data
Moths / virology*
Nucleopolyhedrovirus / genetics*
Open Reading Frames
Restriction Mapping
Selection, Genetic
Sequence Analysis, DNA*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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