| Comparative analysis of fecal microbiota and intestinal microbial metabolic activity in captive polar bears. | |
| | |
MedLine Citation:
|
PMID: 21358758 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The composition of the intestinal microbiota depends on gut physiology and diet. Ursidae possess a simple gastrointestinal system composed of a stomach, small intestine, and indistinct hindgut. This study determined the composition and stability of fecal microbiota of 3 captive polar bears by group-specific quantitative PCR and PCR-DGGE (denaturing gradient gel electrophoresis) using the 16S rRNA gene as target. Intestinal metabolic activity was determined by analysis of short-chain fatty acids in feces. For comparison, other Carnivora and mammals were included in this study. Total bacterial abundance was approximately log 8.5 DNA gene copies·(g feces)-1 in all 3 polar bears. Fecal polar bear microbiota was dominated by the facultative anaerobes Enterobacteriaceae and enterococci, and the Clostridium cluster I. The detection of the Clostridium perfringens α-toxin gene verified the presence of C. perfringens. Composition of the fecal bacterial population was stable on a genus level; according to results obtained by PCR-DGGE, dominant bacterial species fluctuated. The total short-chain fatty acid content of Carnivora and other mammals analysed was comparable; lactate was detected in feces of all carnivora but present only in trace amounts in other mammals. In comparison, the fecal microbiota and metabolic activity of captive polar bears mostly resembled the closely related grizzly and black bears. |
| | |
Authors:
|
Clarissa Schwab; Michael Gänzle |
Related Documents
:
|
3922598 - The effect of osteocalcin on in vitro lipid-induced hydroxyapatite formation and seeded... 19378928 - A comparative analysis of fatty acid composition of root and shoot lipids in zea mays u... 6895738 - Terbutaline and pulmonary surfactant release in the rabbit fetus. 3822258 - Changes in fatty acid composition in myelin lipids after 2,4-dichlorophenoxyacetic buty... 3955538 - Combined effects of butylated hydroxyanisole and other antioxidants in induction of for... 12568348 - Isoforms of soluble alpha-tubulin in oocytes and brain of the frog (genus rana): change... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Canadian journal of microbiology Volume: 57 ISSN: 1480-3275 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
|
Languages: eng Pagination: 177-85 Citation Subset: IM |
Affiliation:
|
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A mutant of Asia 1 serotype of Foot-and-mouth disease virus with the deletion of an important antige...
Next Document: Malate synthase expression is deregulated in the Pseudomonas aeruginosa cystic fibrosis isolate FRD1...