Document Detail

Comparative analysis of extra-ventricular mitoses at early stages of cortical development in rat and human.
MedLine Citation:
PMID:  17717697     Owner:  NLM     Status:  MEDLINE    
Embryonic germinal zones of the dorsal and ventral telencephalon generate cortical neurons during the final week of gestation in rodent and during several months in human. Whereas the vast majority of cortical interneurons originate from the ventral telencephalon, excitatory neurons are locally generated within the germinal zone of the dorsal telencephalon, the future cerebral cortex, itself. However, a number of studies have described proliferating cells external to the ventricular and subventricular germinal zones in the developing dorsal telencephalon. In this study, we performed a comprehensive cell density analysis of such 'extra-ventricular proliferating cells' (EVPCs) during corticogenesis in rat and human using a mitotic marker anti-phospho-histone H3. Subsequently, we performed double-labelling studies with other mitotic and cell type specific markers to undertake phenotypic characterisation of EVPCs. Our findings show: (1) the densities of extra-ventricular H3-positive (H3+) cells were surprisingly similar in preplate stage rat and human; (2) extra-ventricular proliferation continues during mid-and late corticogenesis in rat and in early fetal human cortex; and (3) extra-ventricular cells appear to be mitotic precursors as they are not immunoreactive for a panel of early post-mitotic and cell type-specific markers, although (4) a subset of EVPCs are proliferating microglia. These data suggest that some aspects of early corticogenesis are conserved between rodent and human despite marked differences in the duration of neurogenesis and the anatomical organisation of the developing cerebral cortex.
Rosalind S E Carney; Irina Bystron; Guillermina López-Bendito; Zoltán Molnár
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-02
Journal Detail:
Title:  Brain structure & function     Volume:  212     ISSN:  1863-2653     ISO Abbreviation:  Brain Struct Funct     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-08-24     Completed Date:  2007-10-17     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101282001     Medline TA:  Brain Struct Funct     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  37-54     Citation Subset:  IM    
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MeSH Terms
Biological Markers
Cell Differentiation / physiology*
Cell Movement / physiology
Cell Proliferation*
Cerebral Cortex / cytology,  embryology*,  metabolism*
Histones / analysis,  metabolism
Lateral Ventricles
Mitosis / physiology
Nerve Tissue Proteins / analysis,  metabolism
Neurons / cytology,  metabolism*
Rats, Wistar
Species Specificity
Stem Cells / cytology,  metabolism*
Telencephalon / cytology,  embryology,  metabolism
Grant Support
063974/B/01/Z//Wellcome Trust; G0300200//Medical Research Council
Reg. No./Substance:
0/Biological Markers; 0/Histones; 0/Nerve Tissue Proteins

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