| Comparative analysis of classical and molecular microbiology methods for the detection of Escherichia coli and Enterococcus spp. in well water. | |
| | |
MedLine Citation:
|
PMID: 23014932 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The microbiological quality of 165 1 litre well water samples collected in the Québec City region was assessed by culture-based methods (mFC agar, Chromocult coliform agar, Colilert(®), MI agar, Chromocult enterococci, Enterolert™, and mEI agar) and by a molecular microbiology strategy, dubbed CRENAME-rtPCR, developed for the detection of Escherichia coli, Enterococcus spp., Enterococcus faecalis/faecium, and Bacillus atrophaeus subsp. globigii. In these drinking water samples, approved culture-based methods detected E. coli at rates varying from 1.8 to 3.6% and Enterococcus spp. at rates varying from 3.0 to 11.5%, while the molecular microbiology approach for E. coli was found to be as efficient, detecting contamination in 3.0% of samples. In contrast, CRENAME-rtPCR detected Enterococcus spp. in 27.9% of samples while the E. faecalis/faecium molecular assay did not uncover a single contaminated sample, thereby revealing a discrepancy in the coverage of waterborne enterococcal species detected by classical and molecular microbiology methods. The validation of the CRENAME-E. coli rtPCR test as a new tool to assess the quality of drinking water will require larger scale studies elaborated to demonstrate its equivalence to approved methods. |
| | |
Authors:
|
Andrée F Maheux; Vicky Huppé; Luc Bissonnette; Maurice Boissinot; Lynda Rodrigue; Eve Bérubé; Michel G Bergeron |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
|
Title: Journal of environmental monitoring : JEM Volume: - ISSN: 1464-0333 ISO Abbreviation: J Environ Monit Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100968688 Medline TA: J Environ Monit Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Centre de recherche en infectiologie de l'Université Laval, Centre de recherche du CHUQ, 2705 Laurier Blvd., Québec City, Québec, Canada G1V 4G2. Michel.G.Bergeron@crchul.ulaval.ca. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dehydrin, alcohol dehydrogenase, and central metabolite levels are associated with cold tolerance in...
Next Document: Protective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced...