Document Detail


Comparative analysis of cholinesterase activities in food animals using modified Ellman and Michel assays.
MedLine Citation:
PMID:  22468023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated correlations between modified Ellman and Michel assay methods for measuring cholinesterase (ChE) activities. It also established a foundation for the applicability of measuring ChE activities in food animal species as biochemical biomarkers for evaluating exposure to and effects of organophosphorus and carbamate pesticides. Measuring ChE activities in blood and tissue is currently the most important method of confirming the diagnosis of such exposure. The study also characterized the level of ChE activity in the selected organs/tissues of these animals and determined the best organ/tissue in which to measure ChE activity. The ChE activities were found to be higher in cattle than in sheep and higher in erythrocytes than in plasma and serum. The anticoagulant heparin significantly affects AChE activity in plasma compared with ethylenediamine tetra-acetic acid (EDTA). Of the different tissues tested, the mean of ChE activities was found to be highest in tissue from liver, followed by lung, muscle, kidney, and heart for sheep and cattle. In pigs, the ChE activities tested higher in kidney, liver, lung, muscle, and heart. The highest activities of ChE were found in pigs, followed by cattle and sheep. There was no significant difference between the modified Ellman and Michel method, but the percentage coefficient of variance (%CV) values were higher when the Michel method was used.
Authors:
Kasim Abass Askar; A Caleb Kudi; A John Moody
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire     Volume:  75     ISSN:  0830-9000     ISO Abbreviation:  Can. J. Vet. Res.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2012-04-02     Completed Date:  2012-07-26     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8607793     Medline TA:  Can J Vet Res     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  261-70     Citation Subset:  IM    
Affiliation:
School of Biomedical and Biological Sciences, University of Plymouth, Plymouth, United Kingdom. kasim.abassaskar@plymouth.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / blood,  metabolism*
Animals
Butyrylcholinesterase / blood,  metabolism*
Cattle / metabolism
Kidney / enzymology*
Liver / enzymology*
Lung / enzymology
Muscle, Skeletal / enzymology*
Myocardium / enzymology*
Sheep / metabolism
Species Specificity
Swine / metabolism
Chemical
Reg. No./Substance:
EC 3.1.1.-/Butyrylcholinesterase; EC 3.1.1.7/Acetylcholinesterase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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