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Comparative analysis of cells with combined apoptosis and proliferation markers in thyroid tissue specimens from patients with cancer, adenoma, and autoimmune diseases.
MedLine Citation:
PMID:  22268042     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Combined phenotypes of cells with membrane and intracellular expression of apoptosis and proliferation regulation markers (p53, bcl-2, CD95, CD95L, Ki-67) were studied by flow cytometry of cell suspension from thyroid tissue specimens from patients with autoimmune diseases, adenoma, and thyroid cancer. The incidence of cell groups with phenotypes p53/Ki-67, p53/CD95, bcl-2/Ki-67, bcl-2/CD95, CD95/Ki-67, p53/CD95L, CD95/CD95L, and bcl-2/CD95L was evaluated and the density of receptor distribution on/in each cell group are presented. Patients with autoimmune diseases had high incidence of cells with phenotypes p53/Ki-67, p53/CD95, bcl-2/Ki-67, bcl-2/CD95, CD95/Ki-67, p53/CD95L, CD95/CD95L, and bcl-2/CD95L; cells with the bcl-2/CD95 phenotype were the most incident. Patients with thyroid adenoma had high levels of cells with p53/CD95L phenotype, while patients with thyroid cancer had significantly lower levels of p53 expression in the p53/CD95L cell group. The density of CD95L receptors on CD95/CD95L-positive cells was 4-7-fold higher in patients with thyroid tumors; the density of CD95L receptors on CD95/CD95L cells was maximum in thyroid adenoma and minimum in thyroid cancer. These data indicate differences in the expression of apoptosis and proliferation markers in thyroid adenoma, cancer, and autoimmune diseases. Analysis of the expression of these markers in the above diseases can be useful for differential diagnosis.
Authors:
S P Kazakov; T N Zabotina; O V Korotkova; A K Dzabarov; I A Kazantseva
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  150     ISSN:  1573-8221     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng; rus     Pagination:  453-8     Citation Subset:  IM    
Affiliation:
N. N. Burdenko Military Clinical Hospital, Ministry of Defense of the Russian Federation; N. N. Blokhin Russian Cancer Center, Russian Academy of Medical Sciences, Moscow; M. F. Vladimirsky Moscow Regional Institute, Moscow, Russia. biochimia@mtu-net.ru.
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