Document Detail


Comparative analysis of amino acids and amino-acid derivatives in protein crystallization.
MedLine Citation:
PMID:  20516615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Optimal conditions for protein crystallization are difficult to determine because proteins tend to aggregate in saturated solutions. This study comprehensively evaluates amino acids and amino-acid derivatives as additives for crystallization. This fourth component of the solution increases the probability of crystallization of hen egg-white lysozyme in various precipitants owing to a decrease in aggregation. These results suggest that the addition of certain types of amino acids and amino-acid derivatives, such as Arg, Lys and esterified and amidated amino acids, is a simple method of improving the success rate of protein crystallization.
Authors:
Len Ito; Kentaro Shiraki; Hiroshi Yamaguchi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-27
Journal Detail:
Title:  Acta crystallographica. Section F, Structural biology and crystallization communications     Volume:  66     ISSN:  1744-3091     ISO Abbreviation:  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-02     Completed Date:  2010-11-04     Revised Date:  2012-09-24    
Medline Journal Info:
Nlm Unique ID:  101226117     Medline TA:  Acta Crystallogr Sect F Struct Biol Cryst Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  744-9     Citation Subset:  IM    
Affiliation:
Japan Synchrotron Radiation Research Institute (SPring-8), 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan. l-ito@spring8.or.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*,  chemistry
Animals
Chickens
Crystallization
Muramidase / chemistry*
Chemical
Reg. No./Substance:
0/Amino Acids; EC 3.2.1.-/hen egg lysozyme; EC 3.2.1.17/Muramidase
Comments/Corrections

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