| Comparative analysis of amino acids and amino-acid derivatives in protein crystallization. | |
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MedLine Citation:
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PMID: 20516615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Optimal conditions for protein crystallization are difficult to determine because proteins tend to aggregate in saturated solutions. This study comprehensively evaluates amino acids and amino-acid derivatives as additives for crystallization. This fourth component of the solution increases the probability of crystallization of hen egg-white lysozyme in various precipitants owing to a decrease in aggregation. These results suggest that the addition of certain types of amino acids and amino-acid derivatives, such as Arg, Lys and esterified and amidated amino acids, is a simple method of improving the success rate of protein crystallization. |
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Authors:
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Len Ito; Kentaro Shiraki; Hiroshi Yamaguchi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-27 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 66 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-02 Completed Date: 2010-11-04 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 744-9 Citation Subset: IM |
Affiliation:
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Japan Synchrotron Radiation Research Institute (SPring-8), 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan. l-ito@spring8.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis*,
chemistry Animals Chickens Crystallization Muramidase / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; EC 3.2.1.-/hen egg lysozyme; EC 3.2.1.17/Muramidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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