| Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis. | |
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MedLine Citation:
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PMID: 16201894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ethidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR Gold, SYBR Green, GoldView, GeneFinder, and GoldStar, have been developed. However, there has been no systematic comparative analysis of the staining efficiencies of these dyes. In the present study, SYBR Gold, SYBR Green I, GoldView and EB were compared. Although both SYBR Gold and SYBR Green alter electrophoretic mobility and thus DNA size estimates, they are cost-effective alternatives to EB. SYBR Gold was more sensitive than SYBR Green I at detecting short fragments, but 50-bp bands were clearly visible using either dye when visualized with a long integration time. SYBR Gold or SYBR Green I are sensitive and relatively safe alternatives to EB. In our laboratory, the SYBR Gold method is now used routinely by all members of our group with great consistency and success. |
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Authors:
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Qing Huang; Wei-Ling Fu |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 43 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-10-05 Completed Date: 2005-11-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 841-2 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Southwest Hospital, the Third Military Medical University, Chongqing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cost-Benefit Analysis DNA / analysis* Electrophoresis, Agar Gel / methods* Ethidium / economics, toxicity Fluorescent Dyes* / economics, toxicity Organic Chemicals / economics, toxicity Safety Staining and Labeling / economics, methods* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Organic Chemicals; 0/SYBR Gold nucleic acid gel stain; 163795-75-3/SYBR Green I; 3546-21-2/Ethidium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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