| Comparative analyses of B cell populations in trout kidney and mouse bone marrow: establishing "B cell signatures". | |
| | |
MedLine Citation:
|
PMID: 20705088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study aimed to identify the frequency and distribution of developing B cell populations in the kidney of the rainbow trout, using four molecular B cell markers that are highly conserved between species, including two transcription factors, Pax5 and EBF1, recombination-activating gene RAG1, and the immunoglobulin heavy chain mu. Three distinct B cell stages were defined: early developing B cells (CLP, pro-B, and early pre-B cells), late developing B cell (late pre-B, immature B, and mature B cells), and IgM-secreting cells. Developmental stage-specific, combinatorial expression of Pax5, EBF1, RAG1 and immunoglobulin mu was determined in trout anterior kidney cells by flow cytometry. Trout staining patterns were compared to a well-defined primary immune tissue, mouse bone marrow, and using mouse surface markers B220 and CD43. A remarkable level of similarity was uncovered between the primary immune tissues of both species. Subsequent analysis of the entire trout kidney, divided into five contiguous segments K1-K5, revealed a complex pattern of early developing, late developing, and IgM-secreting B cells. Patterns in anterior kidney segment K1 were most similar to those of mouse bone marrow, while the most posterior part of the kidney, K5, had many IgM-secreting cells, but lacked early developing B cells. A potential second B lymphopoiesis site was uncovered in segment K4 of the kidney. The B cell patterns, or "B cell signatures" described here provide information on the relative abundance of distinct developing B cell populations in the trout kidney, and can be used in future studies on B cell development in other vertebrate species. |
| | |
Authors:
|
Patty Zwollo; Katrina Mott; Maggie Barr |
Related Documents
:
|
20151848 - Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 pro... 15215048 - Sequential induction of hsp25 and proliferating cell nuclear antigen in the kidney afte... 19158348 - Trif is not required for immune complex glomerulonephritis: dying cells activate mesang... 8764978 - Insulin restores the calcium-activated chloride conductance in dedifferentiated renal g... 21110038 - Effects of hyperpolarization-activated cyclic nucleotide-gated (hcn) channel blockers o... 23278248 - The seminiferous epithelial cycle and microanatomy of the koala (phascolarctos cinereus... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2010-08-14 |
Journal Detail:
|
Title: Developmental and comparative immunology Volume: 34 ISSN: 1879-0089 ISO Abbreviation: Dev. Comp. Immunol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-09-24 Completed Date: 2011-01-20 Revised Date: 2013-03-05 |
Medline Journal Info:
|
Nlm Unique ID: 7708205 Medline TA: Dev Comp Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 1291-9 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Biology, The College of William and Mary, Williamsburg, VA 23188, United States. pxzwol@wm.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD43 / analysis Antigens, CD45 / analysis B-Cell-Specific Activator Protein / analysis B-Lymphocytes / immunology*, metabolism Biological Markers / analysis Blotting, Western Bone Marrow / immunology* Cell Differentiation Flow Cytometry Gene Expression Homeodomain Proteins / analysis Immunoglobulin Heavy Chains / analysis Immunoglobulin mu-Chains / analysis Kidney / immunology* Lymphopoiesis* Mice Precursor Cells, B-Lymphoid Trans-Activators / analysis Transcription Factors / genetics Trout / genetics, immunology* |
| Grant Support | |
ID/Acronym/Agency:
|
R15 A1070249-02//PHS HHS; R15 AI070249-01/AI/NIAID NIH HHS; R15 AI070249-02/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD43; 0/B-Cell-Specific Activator Protein; 0/Biological Markers; 0/Ebf1 protein, mouse; 0/Homeodomain Proteins; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin mu-Chains; 0/Pax5 protein, mouse; 0/Trans-Activators; 0/Transcription Factors; 128559-51-3/RAG-1 protein; EC 3.1.3.48/Antigens, CD45 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Concentration regimes and denaturation effects on the conformational changes of ?-chymotrypsin by vi...
Next Document: Pharmacological effect of deoxypodophyllotoxin: a medicinal agent of plant origin, on mammalian neur...