| Comparative LipidomicS Profiling of Human Atherosclerotic Plaques. | |
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MedLine Citation:
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PMID: 21511877 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: -We sought to perform a systematic lipid analysis of atherosclerotic plaques using emerging mass spectrometry techniques. METHODS AND RESULTS: -A chip-based robotic nanoelectrospray platform interfaced to a triple quadrupole mass spectrometer was adapted to analyse lipids in tissue sections as well as extracts from human endarterectomy specimens by shotgun lipidomics. 18 scans for different lipid classes plus additional ones for fatty acids resulted in the detection of 150 lipid species from 9 different classes, of which 24 were detected in endarterectomies only. Further analyses focused on plaques from symptomatic and asymptomatic patients and stable versus unstable regions within the same lesion. Polyunsaturated cholesteryl esters with long chain fatty acids and certain sphingomyelin species showed the greatest relative enrichment in plaques compared to plasma and formed part of a lipid signature for vulnerable and stable plaque areas in a systems-wide network analysis. In principal component analyses the combination of lipid species across different classes provided a better separation of stable and unstable areas than individual lipid classes. CONCLUSIONS: -This comprehensive analysis of plaque lipids demonstrates the potential of lipidomics for unravelling the lipid heterogeneity within atherosclerotic lesions. |
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Authors:
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Christin Stegemann; Ignat Drozdov; Joseph Shalhoub; Julia Humphries; Christophe Ladroue; Athanasios Didangelos; Mark Baumert; Mark Allen; Alun H Davies; Claudia Monaco; Alberto Smith; Qingbo Xu; Manuel Mayr |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-21 |
Journal Detail:
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Title: Circulation. Cardiovascular genetics Volume: - ISSN: 1942-3268 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101489144 Medline TA: Circ Cardiovasc Genet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 King's College London, London, United Kingdom; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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