Document Detail


Comparative effects of probiotics, prebiotics, and synbiotics on growth factors in the large bowel in a rat model of formula-induced bowel inflammation.
MedLine Citation:
PMID:  20683203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Supplementation with probiotics has been shown to prevent gastrointestinal damage possibly through preservation of growth factors. We tested the hypothesis that probiotics, prebiotics, or synbiotics supplementation preserves intestinal insulin-like growth factors (IGFs) and epidermal growth factors (EGFs) in formula-fed neonatal rats.
MATERIALS AND METHODS: At birth (postnatal day 0 [P0]), neonatal rat pups (n = 18 pups/group) were either maternally fed or hand-gavaged with formula supplemented with probiotics (Pro-Fed), prebiotics, or synbiotics from P0 to P3. A formula-fed control group received formula without supplementation. At P4, large bowel samples were assessed histologically and assayed for vascular endothelial growth factor (VEGF), soluble VEGF receptor-1, IGF-I, IGF-II, and EGF.
RESULTS: All formula-fed groups were severely growth suppressed with comparable mortalities. Moderate preservation of bowel integrity was noted in the Pro-Fed group. In contrast, severe inflammation was seen in all of the other formula groups. This was associated with significant increases in VEGF levels in all of the formula groups (P < 0.05) except the Pre-Fed group. Similar elevations in soluble VEGF receptor-1 (P < 0.05), IGF-I (P < 0.05), and EGF (P < 0.05) were noted, but statistical significance was achieved only in the Pro-Fed group.
CONCLUSIONS: Induction of IGF-I and EGF with moderate bowel integrity may represent a protective effect of probiotics against formula-induced inflammation. These data, taken collectively, suggest that probiotics may provide more beneficial effects on the developing large bowel than prebiotics and synbiotics.
Authors:
Lawrence Fordjour; Antoni D'Souza; Charles Cai; Asma Ahmad; Gloria Valencia; Dharmendra Kumar; Jacob V Aranda; Kay D Beharry
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  51     ISSN:  1536-4801     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  507-13     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USA. lfordjour@downstate.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Disease Models, Animal
Epidermal Growth Factor / metabolism
Inflammatory Bowel Diseases / drug therapy*,  metabolism
Insulin-Like Growth Factor I / metabolism
Insulin-Like Growth Factor II / metabolism
Intercellular Signaling Peptides and Proteins / metabolism*
Intestine, Large / metabolism*
Prebiotics*
Probiotics / metabolism,  therapeutic use*
Rats
Rats, Sprague-Dawley
Receptors, Vascular Endothelial Growth Factor / metabolism
Severity of Illness Index
Synbiotics*
Treatment Outcome
Vascular Endothelial Growth Factor A / metabolism
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Prebiotics; 0/Vascular Endothelial Growth Factor A; 62229-50-9/Epidermal Growth Factor; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II; EC 2.7.10.1/Receptors, Vascular Endothelial Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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