Document Detail


Comparative effects of cellulose and soluble fibers (pectin, konjac glucomannan, inulin) on fecal water toxicity toward Caco-2 cells, fecal bacteria enzymes, bile acid, and short-chain fatty acids.
MedLine Citation:
PMID:  20799709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to compare the effects of cellulose and three soluble dietary fibers, pectin, konjac glucomannan (KGM), and inulin, on the cytotoxicity and DNA damage of fecal water-treated Caco-2 cells, a human colon adenocarcinoma cell line, and to investigate the fecal components that potentially modulate the fecal toxicity, that is, bacterial enzymes, bile acids, and short-chain fatty acids. Six-week-old BALB/cJ mice were randomly allocated to consume an AIN-93 diet that contained no dietary fiber (fiber-free) or 5% (w/w) cellulose, pectin, KGM, and inulin for 3 weeks. Feces were collected during days 18-21. Fecal waters were co-incubated with Caco-2 cells to determine the cytotoxicity and DNA damage. In addition, the fecal bacterial enzymes, bile acids, and short-chain fatty acids were determined. Results indicated that all fiber diets similarly increased the survival rate (%) of fecal water-treated Caco-2 cells as compared with the fiber-free diet. The inhibition of fecal water-induced DNA damage in Caco-2 cells was greater for the pectin and inulin diets than for the cellulose and KGM diets. In contrast, cellulose exerted the greatest inhibitory effect on the fecal β-glucuronidase activity. Cellulose and all soluble dietary fibers reduced the secondary bile acid concentrations in the fecal water, but only soluble fibers increased the fecal concentrations of short-chain fatty acids, as compared with no fiber. Therefore, this study suggests that all dietary fibers substantially reduced the fecal water toxicity, which is associated with decreased secondary bile acid levels by all fibers, reduced fecal β-glucuronidase activity by cellulose, and increased short-chain fatty acid levels by soluble dietary fibers.
Authors:
Hsiao-Ling Chen; You-Mei Lin; Yi-Chun Wang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2011-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10277-81     Citation Subset:  IM    
Affiliation:
School of Nutrition, Chung Shan Medical University, and Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan. hlchen@csmu.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / metabolism*
Caco-2 Cells
Cellulose / metabolism
Colorectal Neoplasms / prevention & control
Cytotoxins / toxicity*
Dietary Fiber / metabolism*
Fatty Acids, Volatile / metabolism*,  toxicity
Feces / chemistry*,  enzymology*,  microbiology
Fermentation
Humans
Inulin / metabolism
Male
Mannans / metabolism
Mice
Mice, Inbred BALB C
Pectins / metabolism
Solubility
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cytotoxins; 0/Fatty Acids, Volatile; 0/Mannans; 0/Pectins; 0/konjac gluco-mannan; 9004-34-6/Cellulose; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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