| Comparative effects of cellulose and soluble fibers (pectin, konjac glucomannan, inulin) on fecal water toxicity toward Caco-2 cells, fecal bacteria enzymes, bile acid, and short-chain fatty acids. | |
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MedLine Citation:
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PMID: 20799709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to compare the effects of cellulose and three soluble dietary fibers, pectin, konjac glucomannan (KGM), and inulin, on the cytotoxicity and DNA damage of fecal water-treated Caco-2 cells, a human colon adenocarcinoma cell line, and to investigate the fecal components that potentially modulate the fecal toxicity, that is, bacterial enzymes, bile acids, and short-chain fatty acids. Six-week-old BALB/cJ mice were randomly allocated to consume an AIN-93 diet that contained no dietary fiber (fiber-free) or 5% (w/w) cellulose, pectin, KGM, and inulin for 3 weeks. Feces were collected during days 18-21. Fecal waters were co-incubated with Caco-2 cells to determine the cytotoxicity and DNA damage. In addition, the fecal bacterial enzymes, bile acids, and short-chain fatty acids were determined. Results indicated that all fiber diets similarly increased the survival rate (%) of fecal water-treated Caco-2 cells as compared with the fiber-free diet. The inhibition of fecal water-induced DNA damage in Caco-2 cells was greater for the pectin and inulin diets than for the cellulose and KGM diets. In contrast, cellulose exerted the greatest inhibitory effect on the fecal β-glucuronidase activity. Cellulose and all soluble dietary fibers reduced the secondary bile acid concentrations in the fecal water, but only soluble fibers increased the fecal concentrations of short-chain fatty acids, as compared with no fiber. Therefore, this study suggests that all dietary fibers substantially reduced the fecal water toxicity, which is associated with decreased secondary bile acid levels by all fibers, reduced fecal β-glucuronidase activity by cellulose, and increased short-chain fatty acid levels by soluble dietary fibers. |
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Authors:
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Hsiao-Ling Chen; You-Mei Lin; Yi-Chun Wang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2011-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 10277-81 Citation Subset: IM |
Affiliation:
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School of Nutrition, Chung Shan Medical University, and Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan. hlchen@csmu.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile Acids and Salts / metabolism* Caco-2 Cells Cellulose / metabolism Colorectal Neoplasms / prevention & control Cytotoxins / toxicity* Dietary Fiber / metabolism* Fatty Acids, Volatile / metabolism*, toxicity Feces / chemistry*, enzymology*, microbiology Fermentation Humans Inulin / metabolism Male Mannans / metabolism Mice Mice, Inbred BALB C Pectins / metabolism Solubility |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Cytotoxins; 0/Fatty Acids, Volatile; 0/Mannans; 0/Pectins; 0/konjac gluco-mannan; 9004-34-6/Cellulose; 9005-80-5/Inulin |
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