| Comparative analysis of transduced primary human dendritic cells generated by the use of three different lentiviral vector systems. | |
| | |
MedLine Citation:
|
PMID: 20938757 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Lentiviral gene transfer vectors are suitable for genetically modifying non-cycling primary human cells. In this study, we analyzed transduced human dendritic cells (DC) generated by the use of three different GFP-encoding lentiviral vectors, HIV-2 ROD A Δenv-GFP (ROD A), SIVsmm PBj ΔE EGFP (PBj), and SIVmac ΔE EGFP (SIVmac). CD14(+) monocytes were isolated from buffy coat, transduced, and differentiated to immature and mature DC. Cytofluometric analysis of DC revealed high transduction efficiencies at MOI 1 for simian immunodeficiency virus (SIV)-derived vectors PBj and SIVmac ranging between 80-90 and 70-90%, respectively. In contrast, transduction with ROD A resulted only in approximately 30%-positive DC at the same MOI. Of note, none of the analyzed vectors affected expression of maturation and/or activation markers. Moreover, transduction with PBj or SIVmac did not induce significant cytokine responses whereas ROD A transduction stimulated weak interferon-alpha responses. SIVmac transduced DC showed normal phagocytosis of antigen and normal allo T cell stimulatory capacity when compared with untreated DC. Thus, the SIVmac lentiviral transduction vector is suitable for efficient genetic modification of human DC without affecting phenotype or function and thus qualifies this vector as a versatile tool for use in basic research. |
| | |
Authors:
|
Elena Grabski; Zoe Waibler; Silke Schüle; Björn-Philipp Kloke; Linda Y Sender; Sylvia Panitz; Klaus Cichutek; Matthias Schweizer; Ulrich Kalinke |
Related Documents
:
|
17646737 - Dendritic cells: importance in allergy. 12171547 - Immune modulation by ionizing radiation and its implications for cancer immunotherapy. 14982877 - Thymosin alpha 1 activates dendritic cells for antifungal th1 resistance through toll-l... 21517817 - Inducing apoptosis in chemotherapy-resistant b-lineage acute lymphoblastic leukaemia ce... 16626937 - Apoptosis and schizophrenia: a pilot study based on dermal fibroblast cell lines. 18627477 - Effects of saccharomyces boulardii on cytokine secretion from intraepithelial lymphocyt... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Molecular biotechnology Volume: 47 ISSN: 1559-0305 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 262-9 Citation Subset: IM |
Affiliation:
|
Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, Feodor-Lynen-Str. 7, 30625, Hannover, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative Analysis of Chemokine Receptor's Expression in Mesenchymal Stem Cells Derived from Human...
Next Document: Genetic testing for pheochromocytoma.