Document Detail


Compaction of cell shape occurs before decrease of elasticity in CHO-K1 cells treated with actin cytoskeleton disrupting drug cytochalasin D.
MedLine Citation:
PMID:  19235199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The actin filaments of the cytoskeleton form a highly dynamic polymer scaffold which is actively involved in many essential mechanisms such as cell migration, transport, mitosis, and mechanosensitivity. We treated CHO-K1 cells with different concentrations of the actin cytoskeleton disrupting drug cytochalasin D. Then investigating the cells' elastic behaviour by scanning force microscopy-based rheology we confirmed for high cytochalasin D concentrations (> or =1.5 microM) a significant decrease of mechanical stability. At lower concentrations we measured no significant softening, but flattening and a horizontal contraction was observable even at low concentrations (> or =0.3 microM) of cytochalasin D. The observed changes in cell shape resulted in a lower cell volume, showing that there is compensation by volume for small decreases in cytoskeletal strength resulting from reduced numbers or lengths of actin filaments. These results suggest that the characteristic functions defining a cell's mechanical stability such as mechanosensitivity can be maintained via small changes in cell volume in order to counter fluctuations in cytoskeletal composition.
Authors:
Christian Schulze; Karla Müller; Josef A Käs; Jens C Gerdelmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  66     ISSN:  1097-0169     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-23     Completed Date:  2009-06-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  193-201     Citation Subset:  IM    
Affiliation:
Lehrstuhl für die Physik weicher Materie, Fakultät für Physik und Geowissenschaften, Universität Leipzig, Linnéstrasse 5, Leipzig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
CHO Cells
Cell Line
Cell Shape / drug effects*,  physiology
Cells, Cultured
Cricetinae
Cricetulus
Cytochalasin D / pharmacology*
Cytoskeleton / drug effects*,  metabolism
Dose-Response Relationship, Drug
Elasticity
Microscopy, Atomic Force
Models, Biological
Nucleic Acid Synthesis Inhibitors / pharmacology
Stress, Mechanical
Chemical
Reg. No./Substance:
0/Actins; 0/Nucleic Acid Synthesis Inhibitors; 22144-77-0/Cytochalasin D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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